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Issue title: Selected articles of the 42th Conference of the German Society for Clinical Microcirculation and Hemorheology, 15–16 November 2024, Senftenberg, Germany
Guest editors: S. Braune and F. Jung
Article type: Research Article
Authors: Zeitler, Christinaa; b | Braune, Steffenb; c; * | Waldeck, Petera | Jung, Conrad G.H.a; d | Küpper, Jan-Heinerb; c; d | Petrick, Ingolfa | Jung, Friedrichb
Affiliations: [a] Institute of Biotechnology, Phototrophic Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany | [b] Institute of Biotechnology, Molecular Cell Biology, Brandenburg University of Technology, Cottbus-Senftenberg, Senftenberg, Germany | [c] Faculty of Health Sciences Brandenburg, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany | [d] Carbon Biotech, Social Enterprise Stiftungs AG, Senftenberg, Germany
Correspondence: [*] Corresponding author: Steffen Braune. E-mail: [email protected].
Abstract: The microalgae Arthrospira platensis (AP), commonly known as Spirulina, has gained widespread popularity as a food supplement in recent years. AP is particularly abundant in protein, B vitamins, iron, magnesium, potassium, and various antioxidants. In this study we aimed to evaluate the effect of nitrate limitation in the AP culture medium on AP growth and composition. In addition, the cytotoxicity of the respective aqueous AP extracts on three different mammalian cell-lines (HepG2, Caco2, L929) was tested. AP was cultivated over a 10-day period under nitrogen-rich (Nrich: 1.8 g/L) and nitrogen-deficient (Nlimited: 0.2–0.4 g/L) conditions in two separate experiments, each with three biological replicates (three bioreactors). Throughout the cultivation, the kinetic progress of dry biomass, pH, pigment content, the levels of essential elements (sulphur, phosphate, and nitrate) and the composition of elements in the harvested biomass was determined. While the biomass slightly but significantly differed, the phycocyanin concentration differed considerably (around 10-fold higher in the Nrich medium, p < 0.05). Aqueous extracts of the Nrich medium had significantly stronger effects on the cell membrane integrity and the metabolic activity of the cells than extracts of the Nlimited medium. Particularly was the finding that AP had a significantly stronger toxic effect on the two tumour cell types (HepG2, Caco2) than on the non-tumour cells (L929). This study underscores the significance of nitrate content in the cultivation media of AP.
Keywords: Nitrogen deprivation, culture medium, Arthrospira platensis , biomass productivity, in-vitro cytotoxicity
DOI: 10.3233/CH-248108
Journal: Clinical Hemorheology and Microcirculation, vol. 88, no. s1, pp. S95-S111, 2024
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