Protective effects of Ruscus extract in combination with ascorbic acid and hesperidine methylchalcone on increased leukocyte-endothelial interaction and macromolecular permeability induced by ischemia reperfusion injury

Article type: Research Article

Authors: de Souza, Maria das Graças C.^{; *} | Cyrino, Fatima Z.G.A. | Bouskela, Eliete

Affiliations: Laboratório de Pesquisas Clínicas e Experimentais em Biologia Vascular (BioVasc), Centro Biomédico, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, Brazil

Correspondence: [*] Correspondence to: Maria das Graças C. de Souza, Universidade do Estado do Rio de Janeiro (UERJ), Laboratório de Pesquisas Clínicas e Experimentais em Biologia Vascular (BioVasc), Pavilhão Reitor Haroldo Lisboa da Cunha, térreo- Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro, RJ, 20550-013, Brazil. Tel.: +55 21 23340703, Fax: +55 21 23340692, E-mail: [email protected].

Abstract: BACKGROUND:Despite the well-recognized effectiveness of Ruscus aculetus extract combined or not with ascorbic acid (AA) and hesperidine methyl chalcone (HMC) on ischemia reperfusion (I/R) injury protection, little is known about the contribution of each constituent for this effect. OBJECTIVE:To investigate the effects of AA and HMC combined or not with Ruscus extract on increased macromolecular permeability and leukocyte-endothelium interaction induced by I/R injury. METHODS:Hamsters were treated daily during two weeks with filtered water (placebo), AA (33, 100 and 300 mg/kg/day) and HMC (50, 150 and 450 mg/kg/day) combined or not with Ruscus extract (50, 150 and 450 mg/kg/day). On the day of experiment, the cheek pouch microcirculation underwent 30 min of ischemia, and the number of rolling and adherent leukocytes and leaky sites were evaluated before ischemia and during 45 min of reperfusion. RESULTS:Ruscus extract combined with AA and HMC (Ruscus extract mixture) significantly prevented post-ischemic increase in leukocyte rolling and adhesion and macromolecular permeability compared to placebo and these effects were more prominent than AA and HMC alone on leukocyte adhesion and macromolecular leakage. CONCLUSION:Ruscus extract mixture were more effective than its isolated constituents in protect the hamster cheek pouch microcirculation against I/R injury.

Keywords: Ruscus extract, ischemia/reperfusion injury, hamster cheek pouch preparation, microcirculation

DOI: 10.3233/CH-242111

Journal: Clinical Hemorheology and Microcirculation, vol. Pre-press, no. Pre-press, pp. 1-21, 2024