Investigation of transcriptome profile of ischemia/reperfusion injury of abdominal skin flaps in rats after methane-rich saline treatment using RNA-seq

Article type: Research Article

Authors: Hao, Yan^b | Song, Kexin^{a; *} | Zhang, Mingzi^a | Liu, Hao^b | Qi, Zheng^a | Feng, Cheng^a | Wang, Youbin^a

Affiliations: [a] Department of Plastic Surgery, Peking Union Medical College Hospital, Beijing, China | [b] Department of Plastic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China

Correspondence: [*] Corresponding author: Kexin Song, Department of Plastic Surgery, Peking Union Medical College Hospital, Beijing, 100032, China. Tel.:+86 010 69158730; E-mail: [email protected].

Abstract: OBJECTIVE:Here we examined the influence of methane-rich saline treatment (MS) on the whole transcriptome of the skin flaps during the ischemia/reperfusion (I/R) injuryusing RNA-sequence (RNA-seq). METHODS:The rats were divided into three groups: the sham surgery group (SH),the I/R surgery group treated with physiological saline (I/R-P) or the I/R surgery group treated with the methane-rich saline (I/R-M) respectively. On the 72 hours after operation, the perfusion and the distribution of micro-circulatoryblood flow in skin flaps were observed by laser doppler flowmeters. The whole transcriptome alteration of the skin flaps was examined using RNA-seq. Moreover, the responses of the skin flaps to MRS treatment were examined using bio-informatic and q-PCR approaches after I/R injury. RESULTS:The methane-rich saline (MS) treatment could expand survival area and improve the blood perfusion of the skin flaps after l/R injury. Compared to the I/R-P group, 474 genes significantly altered in the I/R-M group. These genes were mainly associated the development, the cell adhesion and migration. In addition, the PI3K-Akt signal pathway was meaningfully related to regulation of MS treatment. Q-PCR results confirmed that MS treatment positively regulated PI3K-Akt signal pathway relative genes and inhibited the cell adhesion relative genes. CONCLUSION:These results proved that methane-rich saline may alleviate I/R injury and improve flap survival rate by regulating cell adhesion and PI3K-Akt signal pathway.

Keywords: Methane-rich saline, ischemia/reperfusion injury, skin flaps, cell adhesion, PI3K-Akt signal pathway

DOI: 10.3233/CH-201046

Journal: Clinical Hemorheology and Microcirculation, vol. 78, no. 2, pp. 127-138, 2021