Affiliations: [a] Department of Laboratory Medicine, College of Medicine, Korea University Seoul, South Korea
| [b] Division of Cardiology, Internal Medicine, College of Medicine, Korea University Seoul, South Korea
Correspondence:
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Corresponding authors: Hong Seog Seo, MD, Division of Cardiology, Department of Medicine, Guro Hospital, Korea University College of Medicine, Guro 2 Dong, Guro Gu, Seoul 152-703, Korea. Tel.: +82 2 2626 3018; Fax: +82 2 2626 1069; E-mail: [email protected]. and Jung Yoon, MD, Department of Laboratory Medicine, Guro Hospital, Korea University College of Medicine, 148 Guro Dong Ro, Guro Gu, Seoul 08308, Korea. Tel.: +82 2 2626 3242; Fax: +82 2 2626 1465; E-mail: [email protected].
Abstract: BACKGROUND:Platelet function analysis is crucial in assessing the hemostatic status to evaluate congenital and acquired platelet function defects. The Anysis-200 analyzer is a new automated lab-on-a-chip-based platelet function analyzer. OBJECTIVE:We aimed to evaluate a new platelet function analyzing system, the Anysis-200 in comparison to the Platelet Function Analyzer (PFA)-200 in cardiac patients. METHODS:Citrated blood was collected from 174 patients who visited the Department of Cardiology. The Anysis-200 consists of two kits, the microchips with collagen and epinephrine-coated membrane (C/EPI) or adenosine diphosphate-coated membrane (C/ADP). Platelet clogging in the Anysis-200 is measured by the blood migration distance obtained by a camera, which is compatible with the closure time in the PFA-200. We performed Anysis-200 and PFA-200 analyzers simultaneously and compared the results. RESULTS:The sensitivity and specificity of the Anysis-200 C/EPI kit in comparison to the PFA-200 C/EPI kit were 63.41% and 91.43%, respectively. Regarding the C/ADP kit, the sensitivity and specificity of the Anysis-200 were 58.97% and 74.29%, respectively. The agreement rate between the Anysis-200 and PFA-200 for C/EPI was 83.35% and 70.14% for C/ADP. CONCLUSIONS:The Anysis-200, which applies a novel method to detect platelet clogging, has shown moderate to fair agreement with the PFA-200. This test is potentially useful for screening cardiac patients with an abnormal platelet function.
