Affiliations: [a] Heart Institute, University of Pécs, Medical School, Pécs, Hungary
| [b] Department of Immunology and Biotechnology, University of Pécs, Medical School, Pécs, Hungary
| [c] Institute of Bioanalysis, University of Pécs, Medical School, Pécs, Hungary
| [d] Department of Surgical Research and Techniques, University of Pécs, Medical School, Pécs, Hungary
Correspondence:
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Corresponding author: Laszlo Lenard M.D., Heart Institute, University of Pécs, Medical School, H-7624 Pécs, Ifjúság útja 13, Hungary. Tel.: +36 305088636; E-mail: [email protected].
Abstract: BACKGROUND:Composition of pericardial fluid (PF) may reveal immunological processes influencing oxidative stress and microcirculation of different tissues of the heart and may play a role in the course of myocardial infarction, atherosclerosis, and aortic stenosis. PATIENTS AND METHODS:We investigated lymphocyte populations, cardiovascular markers and immunoglobulin composition in PF and blood samples of patients undergoing CABG operation and compared them to those who had aortic valve surgery. RESULTS:The amount of CD8 + T, NK, memoT and activated T-cytotoxic cells were elevated in PF compared to blood, but naiveT and activated T-helper cell ratio were lower in PF. Amount of activated T-helper cells and regulatory T-lymphocytes were elevated in CABG participants in both PF and blood. INKT cells represented the only regulatory lymphocyte population reaching significantly higher concentration in PF than in blood. IL-6 and MCP1 level were elevated in PF compared to blood and MCP1 plasma level was markedly elevated in CABG group. CONCLUSIONS:Our study describes a comprehensive immunological analysis of PF in humans for the first time. We showed that the investigated lymphocyte populations and cardiovascular markers in PF have significantly different distribution compared to blood, and lymphocyte populations show different compartmentization in coronary disease and aortic stenosis.
