Affiliations: [a] Department of Anaesthesiology and Pain Medicine, Korea University, Seoul, Republic of Korea
| [b] College of Medicine, Korea University, Seoul, Republic of Korea
| [c] Department of Biomedical Engineering, College of Medicine, Korea University, Seoul, Republic of Korea
Correspondence:
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Corresponding author: Choon Hak Lim, M.D., Ph.D., Department of Anaesthesiology and Pain Medicine, Korea University Medical Center, 73 Inchon-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea. Tel.: +82 2 920 6639; Fax: +82 2 928 2275; E-mail: [email protected].
Abstract: BACKGROUND: Sepsis is a medical emergency during which early detection is closely associated with mortality. In sepsis, red blood cell (RBC) abnormalities have been reported. However, it is not known how early RBC abnormalities are expressed compared with various clinical manifestations used in sepsis-related organ failure assessment (SOFA). OBJECTIVE: Therefore, using a lipopolysaccharide (LPS)-induced sepsis model we investigated the clinical significance of RBC abnormalities as an early indicator in the detection of septic injury compared with clinical variables. METHODS: Sprague-Dawley rats received LPS (20 mg/kg) intraperitoneally. Aggregation indices (AIs) and aggregation half-time (T1/2), and elongation indices (EI max) were measured. Clinical data-related SOFA and lactate were measured at 2 h, 4 h, 8 h and 12 h after LPS injection. RESULTS: AIs increased at 4 h, and T1/2 decreased at 2 h after LPS injection. Platelet counts decreased at 4 h, and lactate increased at 2 h after LPS injection. AIs showed strong correlations with T1/2 and platelets, EI max increased at 2 h after LPS injection, while EI max had a positive correlation with lactate. CONCLUSIONS: RBC aggregation appears to be an early indicator of clinical deterioration in sepsis and may represent a diagnostic indicator in sepsis.
Keywords: Red blood cell aggregation, red blood cell deformability, sepsis, sepsis-related organ failure assessment
