Modulation of the mesenchymal stem cell migration capacity via preconditioning with topographic microstructure

Issue title: Selected papers of the 36th Conference of the German Society for Clinical Microcirculation and Hemorheology, 5–8. June, 2017, Greifswald, Germany

Guest editors: M. Jünger, A. Krüger-Genge and F. Jung

Article type: Research Article

Authors: Li, Zhengdong^{a; b} | Xu, Xun^{a; b} | Wang, Weiwei^a | Kratz, Karl^{a; d} | Sun, Xianlei^{a; c} | Zou, Jie^{a; b} | Deng, Zijun^{a; b} | Jung, Friedrich^{a; d} | Gossen, Manfred^a | Ma, Nan^{a; b; d; *} | Lendlein, Andreas^{a; b; c; d; *}

Affiliations: [a] Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany | [b] Institute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany | [c] Institute of Biochemistry and Biology, Universität Potsdam, Potsdam, Germany | [d] Helmholtz Virtual Institute “Multifunctional Biomaterials in Medicine”, Teltow, Germany

Correspondence: [*] Corresponding authors: Prof. Dr. Nan Ma and Prof. Dr. Andreas Lendlein, Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513 Teltow. Tel.: +49 3328 352 235; Fax: +49 3328 352 452; E-mail: [email protected] (N. Ma); [email protected] (A. Lendlein).

Abstract: Controlling mesenchymal stem cells (MSCs) behavior is necessary to fully exploit their therapeutic potential. Various approaches are employed to effectively influence the migration capacity of MSCs. Here, topographic microstructures with different microscale roughness were created on polystyrene (PS) culture vessel surfaces as a feasible physical preconditioning strategy to modulate MSC migration. By analyzing trajectories of cells migrating after reseeding, we demonstrated that the mobilization velocity of human adipose derived mesenchymal stem cells (hADSCs) could be promoted by and persisted after brief preconditioning with the appropriate microtopography. Moreover, the elevated activation levels of focal adhesion kinase (FAK) and mitogen-activated protein kinase (MAPK) in hADSCs were also observed during and after the preconditioning process. These findings underline the potential enhancement of in vivo therapeutic efficacy in regenerative medicine via transplantation of topographic microstructure preconditioned stem cells.

Keywords: Mesenchymal stem cells, precondition, microstructure, migration, FAK-MAPK

DOI: 10.3233/CH-179208

Journal: Clinical Hemorheology and Microcirculation, vol. 67, no. 3-4, pp. 267-278, 2017