Ethanol and erythrocyte membrane interaction: a hemorheologic perspective

Article type: Research Article

Authors: Mesquita, R.^; | Gonçalves, M.I. | Dias, S. | Sargento, L. | Saldanha, C. | Martins e Silva, J.

Affiliations: Instituto de Bioquímica, Faculdade de Medicina de Lisboa, Avenida Prof. Egas Moniz, 1600 Lisboa, Portugal

Note: [] Corresponding author. Tel.: +351 1 793 77 04; Fax: +351 1 797 75 78; E‐mail: rmesquit@ fml.fm.ul.pt.

Abstract: Previous studies have documented structural and functional changes induced by ethanol–erythrocyte membrane interaction. In order to perform an in vitro study on the effect of different ethanol concentrations on erythrocyte hemorheologic properties, blood samples were collected from 21 male donors at the Hospital of Santa Maria. Whole blood aliquots were incubated with ethanol solutions of rising concentrations. The following parameters were measured: erythrocyte aggregation, haemoglobin, carboxyhaemoglobin and methaemoglobin concentrations, hematocrit, plasma osmolality and erythrocyte membrane fluidity (fluorescence polarisation probes TMA‐DPH and DPH). With ethanol blood concentrations of 45 mM a rise in plasma osmolality (0.352 Osm/kg H_{2}O vs 0.310 Osm/kg H_{2}O; p<{}0.001) was verified. With 67 mM concentration a decrease of erythrocyte aggregation (11.03 vs 12.81; p<{}0.05) and an increase in plasma osmolality (0.380 Osm/kg H_{2}O vs 0.310 Osm/kg H_{2}O; p<{}0.001) were obtained. In conclusion, ethanol only changes erythrocyte aggregation for a concentration of 67 mM. These data could lead to future changes in therapeutic approaches to situations such as alcoholic coma.

Journal: Clinical Hemorheology and Microcirculation, vol. 21, no. 2, pp. 95-98, 1999