Hemorheological and metabolic consequences of renal ischemia-reperfusion and their modulation by N,N-dimethyl-tryptamine on a rat model

Article type: Research Article

Authors: Peto, Katalin^a | Nemeth, Norbert^{a; *} | Mester, Anita^a | Magyar, Zsuzsanna^a | Ghanem, Souleiman^a | Somogyi, Viktoria^a | Tanczos, Bence^a | Deak, Adam^a | Bidiga, Laszlo^b | Frecska, Ede^{c; 1} | Nemes, Balazs^{d; 1}

Affiliations: [a] Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [b] Department of Pathology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [c] Department of Psychiatry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [d] Division of Organ Transplantation, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

Correspondence: [*] Corresponding author: Norbert Nemeth, MD, PhD, DSc, Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98., Hungary. Tel./Fax: +36 52 416 915; E-mail: [email protected].

Note: [1] These authors contributed equally to the work.

Abstract: BACKGROUND:Micro-rheological relations of renal ischemia-reperfusion (I/R) have not been completely elucidated yet. Concerning anti-inflammatory agents, it is supposed that sigma-1 receptor agonist N,N-dimethyl-tryptamin (DMT) can be useful to reduce I/R injury. OBJECTIVE:To investigate the micro-rheological and metabolic parameters, and the effects of DMT in renal I/R in rats. METHODS:In anesthetized rats from median laparotomy both kidneys were exposed. In Control group (n = 6) no other intervention happened. In I/R group (n = 10) the right renal vessels were ligated and after 60 minutes the organ was removed. The left renal vessels were clamped for 60 minutes followed by 120-minute reperfusion. In I/R+DMT group (n = 10) DMT was administered 15 minutes before the ischemia. Blood samples were taken before/after ischemia and during the reperfusion for testing hematological, metabolic parameters, erythrocyte deformability and aggregation. RESULTS:Lactate concentration significantly increased and accompanied with decreased blood pH. Enhanced erythrocyte aggregation and impaired deformability were observed from the 30th minute of reperfusion. In I/R+DMT group we found diminished changes compared to the I/R group (lactate, pH, electrolytes, red blood cell deformability and aggregation). CONCLUSIONS:Metabolic and micro-rheological parameters impair during renal I/R. DMT could reduce but not completely prevent the changes in this rat model.

Keywords: Kidney, ischemia-reperfusion, hemorheology, N, N-dimethyl-tryptamine

DOI: 10.3233/CH-170361

Journal: Clinical Hemorheology and Microcirculation, vol. 70, no. 1, pp. 107-117, 2018