Comparative evaluation of Plateletworks, Multiplate analyzer and Platelet function analyzer-200 in cardiology patients

Article type: Research Article

Authors: Kim, Jeeyong^a | Cho, Chi Hyun^a | Jung, Bo Kyeung^a | Nam, Jeonghun^a | Seo, Hong Seog^{b; 1; *} | Shin, Sehyun^c | Lim, Chae Seung^{a; 1; *}

Affiliations: [a] Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Republic of Korea | [b] Department of Internal Medicine, Division of Cardiology, College of Medicine, Korea University, Seoul, Korea | [c] School of Mechanical Engineering, Korea University, Seoul, Korea

Correspondence: [*] Corresponding authors. Chae Seung Lim, MD, Department of Laboratory Medicine, Guro Hospital, Korea University College of Medicine, 148 Guro Dong Ro, Guro Gu, Seoul 08308, Korea. Tel.: +82 2 2626 3245; Fax: +82 2 2626 1465; E-mail: [email protected] and Hong Seog Seo, MD, Division of Cardiology, Department of Medicine, Guro Hospital, Korea University College of Medicine, Guro 2 Dong, Guro Gu, Seoul 152-703, Korea, Tel.: +82 2 2626 3018; Fax: +82 2 2626 1069; E-mail: [email protected].

Note: [1] These corresponding authors contributed equally to this work.

Abstract: The objective of this study was to comparatively evaluate three commercial whole-blood platelet function analyzer systems: Platelet Function Analyzer-200 (PFA; Siemens Canada, Mississauga, Ontario, Canada), Multiplate analyzer (MP; Roche Diagnostics International Ltd., Rotkreuz, Switzerland), and Plateletworks Combo-25 kit (PLW; Helena Laboratories, Beaumont, TX, USA). Venipuncture was performed on 160 patients who visited a department of cardiology. Pairwise agreement among the three platelet function assays was assessed using Cohen’s kappa coefficient and percent agreement within the reference limit. Kappa values with the same agonists were poor between PFA-collagen (COL; agonist)/adenosine diphosphate (ADP) and MP-ADP (–0.147), PFA-COL/ADP and PLW-ADP (0.089), MP-ADP and PLW-ADP (0.039), PFA-COL/ADP and MP-COL (–0.039), and between PFA-COL/ADP and PLW-COL (–0.067). Nonetheless, kappa values for the same assay principle with a different agonist were slightly higher between PFA-COL/ADP and PFA-COL/EPI (0.352), MP-ADP and MP-COL (0.235), and between PLW-ADP and PLW-COL (0.247). The range of percent agreement values was 38.7% to 73.8%. Therefore, various measurements of platelet function by more than one method were needed to obtain a reliable interpretation of platelet function considering low kappa coefficient and modest percent agreement rates among 3 different platelet function tests.

Keywords: Multiplate analyzer, Platelet function analyzer-200, Plateletworks

DOI: 10.3233/CH-170331

Journal: Clinical Hemorheology and Microcirculation, vol. 70, no. 3, pp. 257-265, 2018