Affiliations: [a] Southern Medical University, Guangzhou, China
| [b] Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, China
| [c] Department of Anatomy, School of Basic Medicine Sciences, Southern Medical University, Guangzhou, China
| [d] The Graduate School of Third Military Medical University, Chongqing, China
| [e] Center of Wound Treatment, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, China
| [f] The Key Laboratory of Trauma Treatment and Tissue Repair of Tropical Area, PLA, Guangzhou, China
Correspondence:
[*]
Corresponding author: Biao Cheng, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, China. E-mail: [email protected]; Wenhua Huang, Southern Medical University, Guangzhou 510515, China. E-mail: [email protected].
Abstract: BACKGROUND:The effect of lumbar sympathectomy for the treatment of lower limb ischemia remains a matter of controversy. METHODS:Sprague-Dawley rats were subjected to lumbar sympathectomy, after which Evans blue dye was injected into the hind plantar skin. Extravasation of dye was measured and compared with rats undergoing sham operation. Hind plantar skin was processed for HE staining, immunohistochemistry, and Western blot. RESULTS:In sympathectomized rats, blue stained areas in hind plantar skin and concentrations of Evans blue were significantly less than that of sham sympathectomy (control) rats, both 2 weeks and 3 months after surgery. Expression of prostaglandin E2, bradykinin, bradykinin B2 receptor, and adenosine triphosphate were not significantly different between the sympathectomized and control groups. Adenosine receptor A2a expression was significantly reduced in the sympathectomized group both 2 weeks and 3 months after surgery. CONCLUSION:Vascular permeability in the hind plantar skin of rats decreases following lumbar sympathectomy, possibly via reduced expression of adenosine receptor A2a.
