Article type: Research Article
Authors: Kim, Yun Hee | Chung, Hye Gyo | Myung, Soohyun A. | Rha, Jee hyun | Yang, Sung | Nam, Myung Hyun | Shin, Se hyun | Lim, Choon Hak
Affiliations: Department of Anaesthesiology and Pain Medicine, College of Medicine, Korea University, Seoul, Republic of Korea | Department of Medicine, School of Medicine, Korea University, Seoul, Republic of Korea | Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC, USA | Department of Mechatronics, Department of Nanobio Materials and Electronics, Graduate Program of Medical System Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea | Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Republic of Korea | School of Mechanical Engineering, Korea University, Seoul, Republic of Korea
Note: [] Corresponding author: Choon-Hak Lim, M.D., Ph.D., Associate Professor of Anaesthesiology and Pain Medicine, Korea University Medical Center, 126-1 Anam-dong 5-ga, Sungbuk-gu, Seoul, 136-705, Republic of Korea. Tel.: +82 2 920 5632; Fax: +82 2 928 2275; E-mail: [email protected]
Abstract: This in vitro study investigated time-related effects of propofol at the plasma concentrations required for sedation and general anaesthesia, on RBC aggregation, deformability, and morphology. Blood containing propofol at plasma concentrations of 0, 2 and 4 μg ml−1 was incubated in a water bath at 37°C for 1, 2, or 4 hours. RBC elongation indices (EIs) and aggregation indices (AIs), which represent RBC deformability and aggregation, respectively, were measured. Also, RBC morphological indices (MIs), which represent RBC morphology, were calculated. EIs and AIs were similar at propofol concentrations of 0, 2, or 4 μg ml−1 after 1, 2, or 4 hours of incubation. MIs at propofol plasma concentrations 0 or 2 μg ml−1 were similar after 1, 2, and 4 hours of incubation, however, MI at a propofol concentration of 4 μg ml−1 after 4 hours of incubation was higher than its value after 1 or 2 hours of incubation. No significant difference was observed between MIs at propofol plasma concentrations 0, 2, or 4 μg ml−1 after 1, 2, and 4 hours of incubation. At clinical doses, propofol has no direct effects on RBC deformability, aggregation, or morphology over a 4 hours incubation period.
Keywords: Propofol, RBC aggregation, RBC deformability
DOI: 10.3233/CH-2012-1535
Journal: Clinical Hemorheology and Microcirculation, vol. 51, no. 4, pp. 287-292, 2012
