Influence of different radiographic contrast media on the echinocyte formation of human erythrocytes
Issue title: Selected articles of the 30th Annual Conference of the German Society for Clinical Microcirculation and Hemorheology (DGKMH), 18–21 June, 2011, Munich, Germany
Affiliations: Centre for Biomaterial Development and Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Institute of Polymer Research, Helmholtz–Zentrum Geesthacht, Teltow, Germany | Central Institute of Biomedical Engineering, Department of Biomaterials, University of Ulm, Ulm, Germany
Note: [] Corresponding author: Prof. Dr. F. Jung, Institute of Polymer Research, Helmholtz–Zentrum Geesthacht, Teltow, Germany. E-mail: [email protected]
Abstract: Echinocyte formation is associated with a rigidification of the cells that may affect capillary perfusion and, consequently, the tissue oxygen supply. This study examines how many echinocytes appeared after the addition of radiographic contrast media (RCM) (Iodixanol320, Ioversol300, Iopamidol300, and Iomeprol400) compared to red blood cells in autologous plasma and in isotonic saline solution. Isotonic saline solution, Iodixanol, Ioversol, Iopamidol and Iomeprol in concentrations of 10 vol%, 20 vol%, and 40 vol% were added to the plasma of seven healthy subjects. Subsequently, the erythrocytes were resuspended in these plasma/RCM mixtures, incubated for 5 minutes and then examined under the microscope. The concentrations and the RCM in the mixture had a significant effect on the number of discocytes (factor concentration: p < 0.0001; factor RCM: p < 0.0001). The percentage of discocytes for all concentrations depended significantly on the RCM/plasma mixture (concentration × RCM: p < 0.002). Of all RCM/plasma mixtures used, the Iodixanol/plasma mixture showed the most similar discocyte fraction compared to red blood cells in the autologous plasma. Importantly, while Iodixanol differed from all other RCMs, the other RCMs did not differ from one another with respect to the discocyte fraction.
