Angiogenesis: The role of PDGF-BB on Adiopse-tissue derived Stem Cells (ASCs)

Issue title: Selected Presentations from the 29th Conference of the German Society for Clinical Hemorheolgy and Microcirculation, Freie Universität Berlin, Germany, 17–18 September 2010

Article type: Research Article

Authors: Gehmert, Sebastian^{; ;} | Gehmert, Sanga^; | Hidayat, Mulyadi^; | Sultan, Maitham^; | Berner, Arne^; | Klein, Silvan^{; ;} | Zellner, Johannes^; | Müller, Michael^; | Prantl, Lukas^{; ;}

Affiliations: Center of Plastic and Reconstructive Surgery, University Medical Center Regensburg, Regensburg, Germany | Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany | Department of Obstetrics and Gynecology, University Medical Center Regensburg, Regensburg, Germany | Department of Molecular Pathology, The University of Texas, MD, Anderson Cancer Center, Houston, TX, USA

Note: [] Corresponding author. E-mail: [email protected]

Abstract: Recently, it was shown that mesenchymal stem cells (MSCs) are capable of differentiating into endothelial cells which highlights the potential role of MSCs in neovascularization. In the present study, we investigated the paracrine factors responsible for tube formation in human adipose-tissue derived stem cells (ASCs). Moreover, we analyzed ASC's migration towards PDGF-BB and altered levels of proteins involved in different pathways. Freshly isolated human adipose tissue-derived stem cells were seeded onto wells coated with Matrigel and cultured in endothelial growth medium. Capillary-like tube formation was observed after 18 hours culture. Tube formation was significantly reduced in the presence of antibodies against platelet-derived growth factor receptor beta (PDGF) or basic fibroblast growth factor (bFGF). Reverse phase proteomic assay (RPPA) was used to interrogate the expression of 139 phosphorylated or native proteins after incubation with PDGF-BB protein for 24 hours. The present data suggest, that freshly isolated ASCs contain a subpopuplation of stem cells that can form capillary like tubes which is dependent on PDGF and bFGF signaling pathway. Furthermore, Migration of human ASCs significantly increased in response to increased concentrations of PDGF-BB. In addition, incubation of ASCs with PDGF-BB altered phosphorylation of several transcription proteins that are widely expressed throughout the hematopoietic system, targeting genes that have been associated with proliferation, anti-apoptosis or differentiation.

Keywords: Angiogenesis, adipose-tissue derived stem cells, PDGF-BB, endothelial differentiation, migration

DOI: 10.3233/CH-2011-1397

Journal: Clinical Hemorheology and Microcirculation, vol. 48, no. 1-3, pp. 5-13, 2011