Issue title: Selected Proceedings of the 15th Conference of the European Society for Clinical Hemorheology and Microcirculation (ESCHM), June 28–July 1, 2009, Pontresina, Switzerland
Article type: Research Article
Authors: Miko, Iren | Nemeth, Norbert | Sajtos, Erika | Brath, Endre | Peto, Katalin | Furka, Andrea | Szabo, Gyorgyi | Kiss, Ferenc | Imre, Sandor | Furka, Istvan
Affiliations: Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary | Augusta Surgical Center, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary | Third Department of Internal Medicine, Research Group of Gerontology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
Note: [] Corresponding author: Iren Miko, MD, PhD, Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98, Hungary. Tel./Fax: +36 52 416 915; E-mail: [email protected]
Abstract: Erythrocyte deformability could be a suitable parameter reflecting hyposplenic-asplenic conditions after splenectomy or spleen autotransplantation. The aim of this study was to analyze the efficacy of spleen autotransplantation using filtrometry and ektacytometry for testing erythrocyte deformability, besides complex structural, functional, hematological, hemostaseological and immunological examinations. Healthy beagle dogs were subjected to the study in the following experimental groups: “Sham”-sham operated control, “SE”-splenectomy, “AU-5” and “AU-10”-autotransplantation with 5 or 10 spleen chips using “Furka’s spleen chip” technique after splenectomy. Blood samples were taken before the operations and monthly afterwards for 1 year and bi-monthly till the end of the 2nd postoperative year. Erythrocyte deformability was measured by bulk filtrometry (relative cell transit time, RCTT) and on the 20th–24th postoperative months by ektacytometry (elongation index, EI), too. RCTT was elevated in operated groups till the 12th month. On the 20th/24th months “SE”-group expressed the highest level, while the “AU”-groups were close to “Sham”-group. Using ektacytometer we found that on the 20th/24th month both EImax and SS1/2 showed the lowest values (because of unusual curve characteristic) in “SE”-group and “AU”-groups almost reached the “SH”-group. Spleen autotransplantation partially restored the normal rheological conditions. Both filtrometry and ektacytometry was suitable to investigate hyposplenic-asplenic conditions.
Keywords: Splenectomy, spleen autotransplantation, Furka’s spleen chip technique, red blood cell deformability, sepsis
DOI: 10.3233/CH-2010-1307
Journal: Clinical Hemorheology and Microcirculation, vol. 45, no. 2-4, pp. 281-288, 2010
