Affiliations: Third Department of Internal Medicine, Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo 105, JAPAN
Abstract: In this study we attempt to clarify the pathophysiological significance of PFL determined by means of negative pressure filtration system using Nuclepore membrane. The platelet suspension was filtered through the Nuclepore membrane. In the course of filtration pressure difference was generated between the upper and lower sides of membrane due to the PFL. The difference pressure thus generated was detected by a sensitive transducer, variable inductive type, amplified and recorded as a differential pressure curve. The experiment was carried at 37°C in the constant temperature bath. PFL was found to be affected by some factors: 1) A decrease in PFL was observed more significant in suspension of EDTA-platelet compared to that of citrate-platelet. 2) EtTect of ADP on PFL showed to decrease in both suspensions of EDTA and citrate-platelet after addition of ADP in final concentration of 7.5 μmol. However, in the case of citrate-platelet a more significant decrease than that of EDTA-platelet was observed after addition of ADP. 3) Mean platelet volume (MPV). Volume of platelet changed to increase approximately 10% was observed after addition of stimulants such as ADP. 4) PFL in diabetes showed decreasing along with HbA1 concentration. These changes of PFL caused by various factors would contribute to disturbances of microcirculation following microangiopathy.
