Affiliations: School of Life Science and Technology, China Pharmaceutical University, Nanjing, China | State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, China | Hemorheology Center, Department of Biophysics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China
Note: [] Corresponding authors: Wen Zong-yao, Hemorheology Center, Department of Biophysics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. Tel.: +86 10 620 92419; Fax: +86 10 620 15582; E-mail: [email protected]. Prof. Pengfei Tu, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China. Tel./Fax: +86 10 828 02750; E-mail: [email protected].
Abstract: The aim of this study was to test the effects of Ilex kudingcha total saponins on hemorheology of ApoE-/- mice suffering from hypercholesterolemia induced by high-cholesterol diet. The mice were randomly divided into six groups: the control group, the high-cholesterol diet group, 50 mg/kg atorvastatin treatment group, 75, 150 and 300 mg/kg Ilex kudingcha saponins treatment groups, and all the drug treatment groups were fed with a high-cholesterol diet. After administration with saponins (150 mg/kg or more) and atorvastatin (50 mg/kg) for six weeks, the plasma total cholesterol (TC), whole blood viscosity (WBV), plasma viscosity (PV), and erythrocyte aggregation index (EAI) had a remarkable decrease compared with that of the high-cholesterol diet group, but the hematocrit (Hct) and erythrocyte deformation index (DI) had no significant changes. In addition, it is found that the improving effects of saponins on reducing plasma fibrinogen (Fg) levels and prolonging the blood coagulation times including activated partial thromboplastin time (APTT), thrombin time (TT), and prothrombin time (PT). In conclusion, the Ilex kudingcha total saponins may have a significant therapy application of hypercholesterolemia and atherosclerosis by considering its actions on hemorheological characteristics.
Keywords: Hypercholesterolemia, Ilex kudingcha total saponins, hemorheology
