The attenuation effect of 3,4-dihydroxy-phenyl lactic acid and salvianolic acid B on venular thrombosis induced in rat mesentery by photochemical reaction

Article type: Research Article

Authors: Wang, Fang | Liu, Yu-Ying | Liu, Lian-Yi | Zeng, Qing-Jiang | Wang, Chuan-She^; | Sun, Kai | Yang, Ji-Ying | Guo, Jun | Fan, Jing-Yu | Han, Jing-Yan^{; ;}

Affiliations: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China | Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing 100083, China

Note: [] Corresponding author: Jing-Yan Han, Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing 100083, China. E-mail: [email protected].

Abstract: 3,4-dihydroxy-phenyl lactic acid (DLA) and salvianolic acid B (SAB) are two major water-soluble components of Salvia miltiorrhiza (SM). Previous works have revealed the ability of DLA and SAB to scavenge oxygen free radicals, inhibiting the expression of adhesion molecules CD11b/CD18 in neutrophil. Cardiotonic pills (CP), which is a traditional Chinese medicine compound preparation containing DLA and SAB, was found to inhibit venular thrombosis induced by photochemical reaction (PR) in rat mesentery. The present study addressed the effect of DLA and SAB on PR-induced thrombosis in rat mesentery by utilizing a microcirculation dynamic viewing system. The result demonstrated that both DLA and SAB delayed thrombus-initiation time, while DLA also prolonged thrombus half-size time. The experiments explored the mechanism underlying that the dihydrorhodamine 123 (DHR) fluorescence in the mesenteric venular walls after PR challenge was diminished by pretreatment with either DLA or SAB, the expression of CD18 in neutrophils elicited by PR was depressed by administration of DLA, while mast cell degranulation in rat mesentery induced by PR was damped by SAB. The antioxidant potential of the two substances is likely to be responsible for their most beneficial effects on thrombosis, through either directly scavenging the peroxides produced and/or indirectly depressing the expression of adhesion molecules in neutrophil.

Keywords: Antioxidant, adhesion molecule, mast cell degranulation, Salvia miltiorrhiza, thrombosis

DOI: 10.3233/CH-2009-1180

Journal: Clinical Hemorheology and Microcirculation, vol. 42, no. 1, pp. 7-18, 2009