Rheological control preparations for tests of erythrocyte filterability

Article type: Research Article

Authors: Zhu, J.-C. | Stone, P.C.W. | Stuart, J.^;

Affiliations: Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TT, UK

Note: [] Correspondence to: Professor J. Stuart

Abstract: An erythrocyte control preparation for quality assurance purposes would be of considerable value in rheological studies of erythrocyte filterability (deformability). Normal human erythrocytes stored at 4°C in citrate-phosphate-dextrose or saline-adenine-glucose-mannitol appeared to be rheologically stable for 4 weeks when tested using the Hemorheometre or Cell Transit Analyser but, after 2–3 weeks' storage, an increasing sub-population of pore clogging erythrocytes was detected by the St. George's Filtrometer. In contrast, a commercial preparation of stabilised human red blood cells (Coulter SH-RBC) was found to be more stable rheologically, as tested by all three instruments, for up to 6 weeks and thus has potential as a rheological control preparation.

Keywords: Rheology, erythrocyte deformability, quality assurance

DOI: 10.3233/CH-1991-111-213

Journal: Clinical Hemorheology and Microcirculation, vol. 11, no. 1-2, pp. 113-120, 1991