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Fluorescence imaging of Evans blue extravasation into mouse brain induced by low frequency ultrasound with microbubble

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 3rd International Conference on Biomedical Engineering and Biotechnology, 25–28 September 2014, Beijing, China

Article type: Research Article

Authors: Shen, Yuanyuan; ; | Zhang, Aili | Guo, Jinxuan | Dan, Guo; ; | Chen, Siping; ; | Yu, Hao;

Affiliations: Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China | Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Shenzhen 518060, China | National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, Shenzhen 518060, China | School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200240 , China | Department of Biomedical Engineering, Shenzhen Polytechnic, Shenzhen 518055, China

Note: [] Corresponding author: Hao Yu, Department of Biomedical Engineering, Shenzhen Polytechnic, Shenzhen 518055, China. Tel.: +86 075526731493; Fax: +86 075526731493; E-mail: [email protected].

Keywords: Fluorescence imaging, Evans blue, Blood-Brain Barrier disruption, Focused Ultrasound, microbubble

DOI: 10.3233/BME-141101

Journal: Bio-Medical Materials and Engineering, vol. 24, no. 6, pp. 2831-2838, 2014

Published: 2014
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Abstract

Blood-Brain Barrier (BBB) can be opened locally, noninvasively and reversibly by low frequency focused ultra-sound (FUS) in the presence of microbubbles. In this study, Evans blue (EB) dye extravasation across BBB was enhanced by 1 MHz FUS at acoustic pressure of 0.35MPa in the presence of microbubbles at clinically comparable dosage. The spatial distribution of EB extravasation was visualized using fluorescence imaging method. The center region of BBB disruption area showed more enhanced fluorescence signal than the surrounding region in general. However, EB dye deposition was heterogeneous in the center region. The findings in this study indicated potential use of fluorescence imaging to evaluate large molecules delivery across BBB.

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