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Price: EUR 185.00Authors: Barabino, Gilda A. | McIntire, Larry V. | Eskin, Suzanne G. | Udden, Mark M.
Article Type: Research Article
Abstract: The effect of pentoxifylline (PTX) on red blood cell-endothelial cell adherence was studied for sickle and normal red blood cells (RBC). Human umbilical vein endothelial cells grown to confluence on glass slides formed the base of a parallel plate flow chamber into which RBC suspensions were perfused at a constant flow rate, producing a wall shear stress of 1 dyne/cm2 . RBC were incubated with PTX at 37°C for one hour at concentrations ranging from 0.1 to 10.0 mmol/L. Adhesion was monitored using video microscopy. The number of adherent RBC was determined at 10-minute intervals during a wash-out period which …followed a 10-minute perfusion of red cells. When compared with unincubated control RBC, the adherence of sickle RBC incubated with 0.1, 1.0 and 10.0 mmol/L of pentoxifylline was reduced to 65%, 53% and 39% of control values, respectively. There was no significant difference between the adherence of drug incubated and untreated normal RBC. These results indicate that pentoxifylline significantly affects the adhesion of sickle RBC to cultured human endothelial cells, but has no effect on the adherence of normal RBC. Show more
Keywords: Pentoxifylline, sickle cells, endothelial cells, adherence, video microscopy
DOI: 10.3233/CH-1987-7220
Citation: Clinical Hemorheology and Microcirculation, vol. 7, no. 2, pp. 339-349, 1987
Authors: Smith, R.E. | Jones, D.P. | Bowcock, S.A. | Cooke, E.D.
Article Type: Research Article
Abstract: The electrophoretic mobility of erythrocytes from patients with primary Raynaud’s phenomenon was measured in near physiological strength saline using laser Doppler microelectrophoresis and has been shown not to be significantly different from that of matched normal controls. It was found that washing the erythrocytes caused a significant, but small, increase in mobility of up to 3.5%. Two anticoagulation procedures, defibrination and heparinization, were compared and found not to affect measured mobility.
Keywords: Erythrocytes, Raynaud’s Phenomenon, Electrophoretic mobility
DOI: 10.3233/CH-1987-7221
Citation: Clinical Hemorheology and Microcirculation, vol. 7, no. 2, pp. 351-355, 1987
Authors: McMillan, D.E.
Article Type: News
DOI: 10.3233/CH-1987-7222
Citation: Clinical Hemorheology and Microcirculation, vol. 7, no. 2, pp. 357-360, 1987
Authors: Ernst, E.
Article Type: Other
DOI: 10.3233/CH-1987-7223
Citation: Clinical Hemorheology and Microcirculation, vol. 7, no. 2, pp. 361-362, 1987
Article Type: Announcement
DOI: 10.3233/CH-1987-7224
Citation: Clinical Hemorheology and Microcirculation, vol. 7, no. 2, pp. 363-365, 1987
Article Type: Other
DOI: 10.3233/CH-1987-7225
Citation: Clinical Hemorheology and Microcirculation, vol. 7, no. 2, pp. 367-373, 1987
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