Clinical Hemorheology and Microcirculation - Volume 15, issue 1

Authors: Tanahashi, N. | Fukuuchi, Y. | Tomita, M. | Kobari, M. | Takeda, H. | Yokoyama, M. | Itoh, D.

Article Type: Research Article

Abstract: We examined the effect of defibrinogenation with batroxobin on the erythrocyte aggregability (RBC-A) in 16 patients with cerebral infarction during the acute phase (less than 72 hours after onset). Eight patients received a single intravenous administration of 10 units of batroxobin (BU), while the other 8 patients received 5 BU. The RBC-A was examined using the whole-blood erythrocyte aggregometer developed by us (Am. J. Physiol. 251, H1205–H1210, 1986) with concomitant measurement of the hematocrit, albumin:globulin ratio and concentration of fibrinogen. The RBC-A values before, and at 1,2, and 7 days after 10 BU administration were 0.154±0.017/s, 0.117±0.029/s (76% of the …value before the administration, P<0.01), 0.105±0.029/s (68%, P<0.01), and 0.124±0.015/s (81%, P<0.01), respectively. The fibrinogen concentrations before, and at 1, 2, and 7 days after the batroxobin were 364±81 mg/dl, 150±35 mg/dl (41%, P<0.01), 129±35 mg/dl (35%, P<0.01), and 211±42 mg/dl (58%, P<0.01), respectively. On the other hand, the RBC-A values before, and at 1, 2, and 7 days after 5 BU administration were 0.143±0.021/s, 0.131±0.036/s, 0.130±0.028/s, and 0.141±0.029/s, respectively. The fibrinogen concentrations before, and at 1, 2, and 7 days after the batroxobin were 318±57 mg/dl, 255±60 mg/dl (80%, P<0.01), 259±68 mg/dl (81%, P<0.01), and 319±87 mg/dl, respectively. We conclude that a single intravenous administration of batroxobin at 10 BU reduced the RBC-A in patients with cerebral infarction during the acute phase in accordance with a decrease in the fibrinogen level. The effect appeared to last for more than 7 days. Show more

Keywords: batroxobin, defibrinogenation, cerebral infarction, erythrocyte aggregation, hemorheology

DOI: 10.3233/CH-1995-15111

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 89-96, 1995

Authors: Başkurt, Oğuz K. | Edremitlioğlu, Mustafa

Article Type: Research Article

Abstract: Tissue hematocrit was determined in left ventricular myocardium slices in in situ frozen rat hearts and the influence of experimental plasma fibrinogen increments was investigated. Hematocrit values in 100 μm thick myocardial slices were estimated by measuring the activity of two different radionuclides labelling plasma (125 I-Albumin) and red blood cells (99m Tc). In the control group with physiological levels of fibrinogen, myocardial tissue hematocrit was highest at the layer adjacent to epicardium. It decreased linearly through the depth of myocardium, approaching a minimum at the myocardial layer closest to the endocardium. In the group with about two folds higher …plasma fibrinogen concentration, plasma viscosity and red blood cell aggregation were about 50% higher. In this group, tissue hematocrit difference between epicardial and endocardial layers existed, however this difference was more likely due to a step change, rather than a linear gradient. These findings confirm those physiological mechanisms underlying the hematocrit reduction in small blood vessels might be affected by hemorheological alterations. Show more

Keywords: Tissue hematocrit, myocardium, fibrinogen, erythrocyte aggregation

DOI: 10.3233/CH-1995-15112

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 97-105, 1995

Authors: Galaris, Dimitrios | Yova, Dido | Korantzopoulos, Panagiotis | Barbounaki, Stavroula | Koutsouris, Dimitrios

Article Type: Research Article

Abstract: Treatment of human erythrocytes with micromolar concentrations of tert-butyl hydroperoxide causes changes in the physical properties of the cells as evidenced by decreased deformability. The deformability changes followed a lag period which was reciprocally related to the concentrations of hydroperoxide. Ascorbic acid was able to delay these changes or reverse them for a period of time. Oxidation of ascorbic acid was observed, under the same conditions, which was correlated to the ability of both oxy- and met-forms of haemoglobin to catalyse the oxidation of ascorbic acid by tert-butyl hydroperoxide. It seems likely that ascorbic acid acts both as inhibitor of …the oxidative activation of haemoglobin, which leads to deformability changes and as substrate for the haemoglobin catalysed tert-butyl hydroperoxide decomposition. Show more

Keywords: Red Cell Deformability, Tert-butyl hydroperoxide, Ascorbic acid, Human erythrocytes, Cell Transit Analyser

DOI: 10.3233/CH-1995-15113

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 107-120, 1995

Article Type: Book Review

DOI: 10.3233/CH-1995-15114

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 121-121, 1995

Article Type: Other

DOI: 10.3233/CH-1995-15115

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 123-123, 1995

Article Type: Other

DOI: 10.3233/CH-1995-15116

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 125-126, 1995

Article Type: Other

DOI: 10.3233/CH-1995-15117

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 127-127, 1995

Article Type: Other

DOI: 10.3233/CH-1995-15118

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 128-128, 1995

Article Type: Correction

DOI: 10.3233/CH-1995-15119

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 129-129, 1995

Article Type: Other

DOI: 10.3233/CH-1995-15120

Citation: Clinical Hemorheology and Microcirculation, vol. 15, no. 1, pp. 131-131, 1995