Quality control in blood viscoelastometry

Article type: Research Article

Authors: Käßer, U. | Heimburg, P.

Affiliations: Robert-Bosch-Krankenhaus, Department of Cardiology Auerbachstr. 110, 7000 Stuttgart 50, Germany

Note: [] Accepted by: Editor H. Schmid-Schönbein

Abstract: In order to determine accuracy and precision of the oscillatory capillary rheometer (OCR-D), two Newtonian (water, ethyleneglycol) fluids and a Non-Newtonian (aqueous solution of polyacrylamides) fluid were used. In addition, the ranges of normal and pathological plasma viscosity (1.07–4.61 mPas) and of whole blood viscoelasticity (2.94–12.32 mPas for the viscous and 0–6.46 mPas for the elastic component) were determined in order to select the ranges of viscoelastic parameters for control materials of clinical interest. Mixtures of water and ethyleneglycol were examined to prove their suitability as control samples for plasma viscosity: mixtures in a ratio of 1 to 0.5 and 1 to 4 (v/v) are recommended. To control the measurement of viscoelastic parameters of whole blood, the Non-Newtonian fluid (PAA) and a commercially available red blood cell test suspension (Dia-HT2) were investigated for their precision parameters. For quality control of the viscous component both solutions are required, whereas PAA may also serve for control of the elastic component, Dia-HT2 is not appropriate due to very low elastic responses.

Keywords: blood viscoelasticity, plasma viscosity, quality control, oscillatory capillary rheometer

DOI: 10.3233/CH-1988-8115

Journal: Clinical Hemorheology and Microcirculation, vol. 8, no. 1, pp. 93-103, 1988