Affiliations: [a] Centre de Transfusion Sanguine, 44000 Nantes, France | [b] Clinique Médicale B, 44000 Nantes, France | [c] I.N.R.A, Laboratoire de Biochimie des Protéines, 44000 Nantes, France
Note: [1] Part of this paper was presented at the 2nd European Conference on Clinical Haemorheology. London, UK (1981)
Note: [] Accepted by: Editor J.F. Stoltz
Abstract: This study was undertaken to assess the role of glycosylated hemoglobins (HbA1) in the reduced erythrocyte deformability of diabetic patients. Filtration tests were carried out on washed RBC suspensions and data show no correlation between the relative filtration time (RFT) and cell HbA1 concentration. Conversely, a strong correlation was found between RFT an cell HbA0 concentration. On the other hand, viscometric assays were performed on hemoglobin solutions after HbA1a+b, HbA1c and HbA0 were separated by chromatography. Glycosylated hemoglobin fractions show intrinsic viscosities about 2.5 fold that of non glycosylated fractions (HbA1a+b : {η} = 7.12 ml/g, HbA1c : {η} = 7.63 ml/g, HbA0 : {η} = 3.07 ml/g). In conclusion, our data suggest that an increase in HbA1 induces an increase in RBC internal viscosity but seems to have no influence on RBC deformability when tested with a 5 µm filter.
