Brillouin confocal microscopy to determine biomechanical properties of SULEEI-treated bovine pericardium for application in cardiac surgery

Issue title: 40th Conference of the German Society for Clinical Microcirculation and Hemorheology, 5-6 November 2021, Senftenberg, Germany

Guest editors: J.-H. Küpper, A. Krüger-Genge and F. Jung

Article type: Research Article

Authors: Jannasch, Anett^{a; 1; *} | Rix, Jan^{b; 1} | Welzel, Cindy^{a; 1} | Schackert, Gabriele^d | Kirsch, Matthias^{d; e} | König, Ulla^f | Koch, Edmund^b | Matschke, Klaus^a | Tugtekin, Sems Malte^a | Dittfeld, Claudia^{a; 1} | Galli, Roberta^{c; 1}

Affiliations: [a] Department of Cardiac Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Heart Centre Dresden, Dresden, Germany | [b] Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Dresden, Germany | [c] Department of Medical Physics and Biomedical Technology, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Dresden, Germany | [d] Neurosurgery, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany | [e] Klinik für Neurochirurgie, Asklepios Kliniken Schildautal, Seesen, Germany | [f] Department of Medical and Biotechnological Applications, Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology, Dresden, Germany

Correspondence: [*] Corresponding author: Dr. Anett Jannasch, Department of Cardiac Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Heart Centre Dresden, Fetscherstraße 76, 01307 Dresden, Germany. Tel.: +49 351 450 1090; Fax: +49 351 450 1802; E-mail: [email protected].

Note: [1] These authors contributed equally.

Abstract: BACKGROUND:Heart valves are exposed to a highly dynamic environment and underlie high tensile and shear forces during opening and closing. Therefore, analysis of mechanical performance of novel heart valve bioprostheses materials, like SULEEI-treated bovine pericardium, is essential and usually carried out by uniaxial tensile tests. Nevertheless, major drawbacks are the unidirectional strain, which does not reflect the in vivo condition and the deformation of the sample material. An alternative approach for measurement of biomechanical properties is offered by Brillouin confocal microscopy (BCM), a novel, non-invasive and three-dimensional method based on the interaction of light with acoustic waves. OBJECTIVE:BCM is a powerful tool to determine viscoelastic tissue properties and is, for the first time, applied to characterize novel biological graft materials, such as SULEEI-treated bovine pericardium. Therefore, the method has to be validated as a non-invasive alternative to conventional uniaxial tensile tests. METHODS:Vibratome sections of SULEEI-treated bovine pericardium (decellularized, riboflavin/UV-cross-linked and low-energy electron irradiated) as well as native and GA-fixed controls (n = 3) were analyzed by BCM. In addition, uniaxial tensile tests were performed on equivalent tissue samples and Young’s modulus as well as length of toe region were analyzed from stress-strain diagrams. The structure of the extracellular matrix (ECM), especially collagen and elastin, was investigated by multiphoton microscopy (MPM). RESULTS:SULEEI-treated pericardium exhibited a significantly higher Brillouin shift and hence higher tissue stiffness in comparison to native and GA-fixed controls (native: 5.6±0.2 GHz; GA: 5.5±0.1 GHz; SULEEI: 6.3±0.1 GHz; n = 3, p < 0.0001). Similarly, a significantly higher Young’s modulus was detected in SULEEI-treated pericardia in comparison to native tissue (native: 30.0±10.4 MPa; GA: 31.8±10.7 MPa; SULEEI: 42.1±7.0 MPa; n = 3, p = 0.027). Native pericardia showed wavy and non-directional collagen fibers as well as thin, linear elastin fibers generating a loose matrix. The fibers of GA-fixed and SULEEI-treated pericardium were aligned in one direction, whereat the SULEEI-sample exhibited a much denser matrix. CONCLUSION:BCM is an innovative and non-invasive method to analyze elastic properties of novel pericardial graft materials with special mechanical requirements, like heart valve bioprostheses.

Keywords: Biomechanics, Brillouin confocal microscopy, heart valve prosthesis, Young’s modulus

DOI: 10.3233/CH-219119

Journal: Clinical Hemorheology and Microcirculation, vol. 79, no. 1, pp. 179-192, 2021