Effect of HBO therapy on adipose-derived stem cells, fibroblasts and co-cultures: In vitro study of oxidative stress, angiogenic potential and production of pro-inflammatory growth factors in co-cultures¹

Issue title: Selected post-conference papers of the 39th Conference of the German Society for Clinical Microcirculation and Hemorheology, 6-7 November 2020, Hannover, Germany

Article type: Research Article

Authors: Engel, P.^a | Ranieri, M.^a | Felthaus, O.^{a; *} | Geis, S.^a | Haubner, F.^d | Aung, T.^a | Seyfried, T.^c | Prantl, L.^a | Pawlik, MT.^b

Affiliations: [a] Department of Plastic, Aesthetic, Hand and Reconstructive Surgery, University Hospital, University of Regensburg, Regensburg, Germany | [b] Department of Anesthesiology and Intensive Care Medicine, St. Josef Hospital Regensburg, Regensburg, Germany | [c] Department of Anesthesiology and Intensive Care Medicine, University Hospital, University of Regensburg, Regensburg, Germany | [d] Department of Otorhinolaryngology, University of Munich, Munich, Germany

Correspondence: [*] Corresponding author: Oliver Felthaus, Department of Plastic und Aesthetic, Hand and Reconstructive Surgery, University Hospital, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany. Tel.: +49 941 9446763; E-mail: [email protected].

Note: [1] This article was presented at the 39th Conference of the German Society for Clinical Microcirculation and Hemorheology, 6-7 November 2020, Hannover, Germany.

Abstract: BACKGROUND:A key moderator of wound healing is oxygen. Wound healing is a dynamic and carefully orchestrated process involving blood cells, cytokines, parenchymal cells (i.e. fibroblasts and mesenchymal stem cells) and extracellular matrix reorganization. Human adipose derived stem cells as well as human fibroblasts produce soluble factors, exhibit diverse effects on inflammation and anti inflammation response and are involved in wound healing processes.Hyperbaric oxygen therapy is an effective adjunct treatment for ischemic disorders such as chronic infection or chronic wounds. In vitro effects of hyperbaric oxygen therapy on human cells were presented in many studies except for those on mono- and co-cultures of human adipose derived stem cells and fibroblasts. OBJECTIVE:The aim of this study was to investigate the effects of hyperbaric oxygen therapy on mono- and co-cultures of human adipose derived stem cells and fibroblasts. METHODS:Mono- and co-cultures from human adipose derived stem cells and fibroblasts were established. These cultures were exposed to hyperbaric oxygen therapy every 24 h for five consecutive days. Measuring experiments were performed on the first, third and fifth day. Therapy effects on the expression of VEGF, IL 6 and reactive oxygen species were investigated. RESULTS:After exposure to hyperbaric oxygen, cell culturess showed a significant increase in the expression of VEGF after 3 and 5 days. All cultures showed significantly reduced formation of reactive oxygen species throughout the experiments. The expression of IL-6 decreased during the experiment in mono-cultures of human adipose derived stem cells and co-cultures. In contrast, mono-cultures of human skin fibroblasts showed an overall significantly increased expression of IL-6. CONCLUSIONS:Hyperbaric oxygen therapy leads to immunmodulatory and proangiogenetic effects in a wound-like enviroment of adipose derived stem cells and fibroblasts.

Keywords: HBO therapy, adipose derived stem cells, cell oxygenation, cell co-culture, angiogenesis, hyperbaric oxygen

DOI: 10.3233/CH-209222

Journal: Clinical Hemorheology and Microcirculation, vol. 76, no. 4, pp. 459-471, 2020