Affiliations: [a]
Department of Plastic and Hand Surgery, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (FAU), Erlangen, Germany
| [b]
Institute of Medical Biotechnology, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany
| [c]
Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany
| [d]
Chair of Pathology and Anatomy, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany
| [e]
Department of Paediatric Cardiac Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany
| [f]
Department of Anesthesiology, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany
Correspondence:
[*]
Corresponding author: Christian D. Taeger, M.D., Department of Plastic and Hand Surgery, Director: Univ. Prof. Dr. med. Dr. h. c. Raymund E. Horch, University Hospital of Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Krankenhausstr. 12, 91054 Erlangen, Germany. Tel.: +49 9131 8542168; Fax: +49 9131 8539327; E-mail: [email protected].
Abstract: In the field of free flap transfer in reconstructive surgery, the trans- or replanted tissue always undergoes cell damage during ischemia to a more or less strong extent. In previous studies we already showed that conserving muscle transplants by means of extracorporeal perfusion over a period of 6 hours by using a crystalloid solution for perfusion. However, we observed significant edema formation. In this study we aimed at reducing the edema formation by using an iso-oncotic colloid as perfusion solution. This way we wanted to evaluate a possible new application of hydroxyl-ethyl starch in an extracorporeal setup to exploit potential benefits of the colloid. Examined parameters include the muscles’ functionality with external field stimulation, histological examination and edema formation. Perfused muscles showed a statistically significant higher ability to exert force compared to nonperfused ones. These findings can be confirmed using Annexin V as marker for cell damage, as perfusion of muscle tissue limits damage significantly compared to nonperfused tissue. Substituting the electrolyte perfusion solution with a colloidal one shows the tendency to reduce the edema formation however without statistical significance.
