The effect of diabetic ketoacidosis (DKA) and its treatment on clot microstructure: Are they thrombogenic?

Article type: Research Article

Authors: Pillai, Suresh^{a; b; c} | Davies, Gareth^a | Lawrence, Matthew^{a; c} | Whitley, Janet^{a; c} | Stephens, Jeffrey^{b; c} | Williams, Phylip Rhodri^c | Morris, Keith^d | Evans, Phillip Adrian^{a; b; c; *}

Affiliations: [a] Welsh Centre for Emergency Medicine Research, Emergency Department, Morriston Hospital, Swansea, UK | [b] Morriston Hospital, Swansea, UK | [c] Swansea University, Swansea, UK | [d] Cardiff Metropolitan University, UK

Correspondence: [*] Corresponding author: Phillip Evans, Welsh Centre for Emergency Medicine Research Morriston Hospital Swansea, Swansea, SA6 6NL UK. E-mail: [email protected].

Abstract: BACKGROUND:Diabetic ketoacidosis (DKA) is a medical emergency with a high mortality rate and is associated with severe metabolic acidosis and dehydration. DKA patients have an increased risk of arterial and venous thromboembolism, however little is known about this metabolic derangement in the first 24 hours of admission and to assess its effect on coagulation. We therefore utilised a novel functional marker of clot microstructure (fractal dimension - df) to assess these changes within the first 24 hours. METHODS:Prospective single centre observational study to demonstrate whether the tendency of blood clot formation differs in DKA patients. RESULTS:15 DKA patients and 15 healthy matched controls were recruited. Mean df in the healthy control group was 1.74±0.03. An elevated df of 1.78±0.07 was observed in patients with DKA on admission. The mean pH on admission was 7.14±0.13 and the lactate was 3.6±2.0. df changed significantly in response to standard treatment and was significantly reduced to 1.68±0.09 (2–6& h) and to 1.66±0.08 at 24& h (p < 0.01 One-way ANOVA). df also correlated significantly with lactate and pH (Pearson correlation coefficient 0.479 and –0.675 respectively, p < 0.05). CONCLUSIONS:DKA patients at presentation have a densely organising less permeable thrombogenic clot microstructure as evidenced by high df. These structural changes are due to a combination of dehydration and a profound metabolic acidosis, which was reversed with treatment. These changes were not mirrored in standard clinical markers of thromboge-nicity.

Keywords: Diabetic ketoacidosis (DKA), haemorheology, fractal dimension, gel point

DOI: 10.3233/CH-200957

Journal: Clinical Hemorheology and Microcirculation, vol. 77, no. 2, pp. 183-194, 2021