Red blood cell aggregation, disaggregation and aggregate morphology in autologous plasma and serum in diabetic foot disease

Article type: Research Article

Authors: Sheremet’ev, Yury A.^{; *} | Popovicheva, Aleksandra N. | Rogozin, Methun M. | Levin, Grigory Ya.

Correspondence: [*] Corresponding author: Yury A. Sheremet’ev, Ph.D., Department of gravitation surgery and hemodialisys, FSBEI HE «Privolzhsky Research Medical University» of the Ministry of Health of the Russian Federation, 10/1, Minin and Pozharsky Sq., 603005, Nizhny Novgorod, Russian Federation. Tel.: +8 831 432 17 58; E-mail: [email protected].

Abstract: BACKGROUND:Diabetes mellitus is frequently associated with microcirculation pathology and hemorheological disorders. METHODS:24 patients with diabetic foot and 22 healthy subjects were recruited. RBC aggregation, disaggregation and morphology of aggregates were determined in autologous plasma and serum. RESULTS:The RBC aggregation in patients with diabetic foot increased in autologous plasma and serum. Increased red blood cell aggregate strength in these patients was observed only in autologous plasma. Microscopic images of RBC aggregates of patients with diabetic foot show the formation of pathologic globular structures of aggregates in autologous plasma and serum. CONCLUSION:The RBC aggregation in autologous plasma and autologous serum in patients with diabetic foot is significantly higher than in healthy subjects. Increase in strength of RBC aggregates in diabetic foot patients was observed only in autologous plasma. The microscopic images of RBC aggregates in patients with diabetic foot indicate the formation of globular (pathologic) structures of aggregates in autologous plasma and serum. The differences in the morphology of RBC aggregates in autologous plasma and serum between healthy subjects and diabetic foot patients, obtained by microscopic image analysis with high magnification light microscope, can be used as an additional diagnostic tool in medical practice.

Keywords: Diabetic foot, red blood cell aggregation, disaggregation, aggregates morphology, autologous plasma, autologous serum

DOI: 10.3233/CH-180405

Journal: Clinical Hemorheology and Microcirculation, vol. 72, no. 3, pp. 221-227, 2019