Affiliations: [a]
Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, UK
| [b]
School of Biosciences, Cardiff University, Cardiff, UK
| [c]
School of Earth and Ocean Sciences, Cardiff University, Cardiff, UK
Correspondence:
[*]
Corresponding author: Dr Rachel A. Adams, Cardiff School of Health Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff, CF5 2YB, UK. Tel.: +44 029 20416839; Fax: +44 029 20416982; E-mail: [email protected].
Abstract: BACKGROUND:Exposure to air pollution is associated with cardiovascular disease, including increased morbidity and mortality rates. OBJECTIVE:The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on biomarkers of leucocyte activation and vascular endothelial damage. METHODS: Air pollution particles (PM10) were instilled into rats, and blood samples were taken three days and six weeks post instillation. Plasma neutrophil elastase and Von Willebrand factor were measured by ELISA. RESULTS: Plasma neutrophil elastase increased from 175±44 ng/ml at baseline to 288±26 ng/ml 3 days post instillation (p = 0.038). vWF increased from 0.160±0.015 IU/ml at baseline to 0.224±0.015 IU/ml at 3 days post and 0.208±0.01 IU/ml at 6 weeks post (p = 0.006, ANOVA). sICAM-1 increased from 17.75±0.70 ng/ml at baseline to 19.03±0.33 ng/ml at 3 days post and 21.72±1.16 ng/ml at 6 weeks post (p = 0.009, ANOVA). CONCLUSION: Instillation caused prolonged systemic inflammation, activation of blood leucocytes and damage to the vascular endothelium.
Keywords: Particles, cardiovascular disease, inflammation, endothelial damage and leucocyte
