Affiliations: [a] Department of Neonatal Intensive Care, Oslo University Hospital, Ullevål, Oslo, Norway | [b] Faculty of Medicine, University of Oslo, Oslo, Norway | [c] Department of
Cardiothoracic Surgery, Oslo University Hospital, Ullevål, Oslo, Norway | [d] Department of
Biomedical Engineering, Linköping University, Linköping, Sweden
Correspondence:
[*]
Correspondence to: Siv Fredly, Department of Neonatal Intensive Care, Oslo University Hospital, Ullevål,
Oslo, Norway. [email protected]
Abstract: Despite microcirculation’s fundamental role, assessments of its function are limited. We explored the applicability of Computer Assisted Video Microscope (CAVM), Laser Doppler Perfusion Measurements (LDPM) and Diffuse Reflectance Spectroscopy (DRS) to study skin microvascular morphology, perfusion and oxygen saturation in twenty-five healthy newborns day 1–3 of life. Results: Day 1–3 (mean (SD)): Microvascular density (CAVM; number of microvessels crossing a grid of lines/mm line, c/mm): Chest: 11.3 (1.5), 11.0 (1.7), 10.7 (1.6). Hand: 13.2 (2.0), 13.2 (1.9), 12.4 (1.6). Capillary density was significantly higher in the hand than in the chest each day (p < 0.001). Perfusion (LDPM; arbitrary units): Chest: 109.1 (26.0), 101.4 (24.6), 100.8 (25.3). Hand: 58.9 (17.5), 54.3 (15.8), 46.9 (14.8). Perfusion was significantly higher in the chest than in the hand each day (p < 0.01). Microvascular oxygen saturation (DRS; %): Chest: 88.1 (5.2), 87.8 (10.0), 86.7 (9.0). Hand: 79.9 (15.2), 82.7 (11.8), 82.2 (12.1) (p < 0.05). Capillary flow velocities (CAVM) were similar in the chest and hand: 60–70% capillaries had “continuous high flow” and 30–40% “continuous low flow”. Multimodal skin microvascular assessments with CAVM, LDPM and DRS are feasible with reproducible data in newborns. The hand has lower perfusion, higher capillary density and higher oxygen extraction than the chest.
