Characterization of thoracal fat depots – expression of adipokines and remodeling factors and impact of adipocyte conditioned media in fibroblast scratch assays

Article type: Research Article

Authors: Dittfeld, C.^{; 1; *} | Bienger, K.^{; 1} | Andres, J.^{; 1} | Plötze, K. | Jannasch, A. | Waldow, T. | Tugtekin, S.M. | Matschke, K.

Affiliations: Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Herzzentrum Dresden, Germany

Correspondence: [*] Corresponding author: Dr. Claudia Dittfeld, Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Herzzentrum Dresden, Fetscherstr. 76, 01307 Dresden, Germany. Tel.: +49 0351 450 1090; Fax: +49 0351 450 1707; E-mail: [email protected].

Note: [1] These authors contributed equally to this work.

Abstract: Adipose tissue is not only a connective tissue but also an endocrine organ secreting adipokines like Leptin and Adiponectin, lipokines such as palmitoileic acid and extracellular vesicles. These factors and the expression of matrix remodeling enzymes impact surrounding tissues via paracrine effects. The expression of selected secretion factors and the effect of adipocyte conditioned media from four thoracal adipose tissue origins - subcutaneous, perivascular, pericardial and epicardial adipose tissues – in a fibroblast proliferation/wound healing scratch assay model were investigated. Results were compared directly and according to the type 2 diabetic mellitus (T2DM) status of the patients the tissues are originated from. Adipocyte conditioned media from non-diabetic patients resulted in a significant higher scratch closure rate compared to the media with T2DM background. Linoleic acid incubation in scratch assay resulted in a reduced scratch closure rate. Leptin, Adiponectin and Visfatin/Nampt expression and MMP2, MMP9 and FSTL1 mRNA levels did not vary according to T2DM subgroups directly, leading to the assumption that these factors are not causal for scratch assay effects observed. In contrast significant mRNA expression differences were monitored between the thoracal tissue origins implying variations in the local effects of the different adipose tissue depots.

Keywords: Thoracal adipose tissue depots, adipokines, MMP, scratch wound healing assay, fatty acids, type 2 diabetes mellitus

DOI: 10.3233/CH-170341

Journal: Clinical Hemorheology and Microcirculation, vol. 70, no. 3, pp. 267-280, 2018