Leukocyte-reduced platelet-rich plasma stimulates the in vitro proliferation of adipose-tissue derived mesenchymal stem cells depending on PDGF signaling

Article type: Research Article

Authors: Lang, Siegmund^{a; *} | Herrmann, Marietta^{b; c} | Pfeifer, Christian^a | Brockhoff, Gero^d | Zellner, Johannes^a | Nerlich, Michael^a | Angele, Peter^a | Prantl, Lukas^e | Gehmert, Sebastian^{a; e; f} | Loibl, Markus^a

Affiliations: [a] Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany | [b] AO Research Institute, Davos, Switzerland | [c] IZKF Group Tissue Regeneration in Musculoskeletal Diseases, Orthopedic Center for Musculoskeletal Research, University Würzburg, Würzburg, Germany | [d] Department of Obstetrics and Gynecology, University Medical Center Regensburg, Regensburg, Germany | [e] Center of Plastic, Hand and Reconstructive Surgery, University Medical Center Regensburg, Regensburg, Germany | [f] Department of Orthopedic Surgery, University Hospital Basel, Basel, Switzerland

Correspondence: [*] Corresponding author: Siegmund Lang, Department of Trauma Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, Regensburg, Germany. Tel.: +49 0 941 944 6842; E-mail: [email protected].

Abstract: BACKGROUND:Platelet-rich Plasma (PRP) is suggested as xenoprotein-free cell-culture medium replacement for animal-derived supplements. OBJECTIVE:The aim of this study was to investigate PRP-triggered signaling in adipose derived mesenchymal stem cells (ASCs). METHODS:PRP was obtained from 4 male patients. We incubated ASCs in α-MEM with different Platelet derived growth factor (PDGF) subtypes or 10% or 20% pooled PRP or 20% fetal calf serum (FCS) prior to determination of the S-phase fraction (SPF). To investigate the influence of PDGF signaling on ASCs, PDGF receptor β inhibitor was added, and protein expression of ASCs was measured. RESULTS:ASCs exposed to 20% PRP, PDGF-AB and – BB demonstrated significant higher SPF in comparison to PDGF-AA and 20% FCS after 48 hours (all P < 0.05). PDGF receptor β inhibition diminished the PRP-induced SPF increase of ASCs significantly after 48 hours (P < 0.01). ASCs with PDGF receptor β inhibition showed significant higher PDGF receptor β and significant lower c-MYC expression compared to untreated cells in presence of 20% PRP after 48 hours (both P < 0.05). CONCLUSIONS:The proliferation promoting effect of PRP on ASCs is mediated by PDGF signaling and is associated with c-MYC overexpression.

Keywords: Platelet-rich Plasma, mesenchymal stem cells, PDGF receptor β inhibition, proliferation, cell culture

DOI: 10.3233/CH-170246

Journal: Clinical Hemorheology and Microcirculation, vol. 67, no. 2, pp. 183-196, 2017