Affiliations: Department Medical Physics and Biophysics, Medical University of Sofia, Bulgaria
Correspondence:
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Corresponding author: Svetoslav Jovtchev, Department Medical Physics and Biophysics, Medical Faculty, Medical University of Sofia, Street Zdrave 2, 1431 Sofia, Bulgaria. Tel.: +359 2 9172 696; E-mail: [email protected].
Abstract: BACKGROUND: Different colloids are used as a part of solutions for fluid resuscitation and organ preservation: hydroxyethyl starches (HES), dextran (Dx), polyethylene glycols (PEG), polyvinyl pyrrolidone (PVP). Some of the problems associated with their application are addressed to alteration in erythrocyte (ERY) rheology. OBJECTIVE: We intended to estimate in vitro and compare the aggregation power (AP) of these molecules related to ERY interactions. METHODS: Washed human ERY are used during the study. The zeta sedimentation technique is used to quantify the cell aggregation. Zeta sedimentation ratio (ZSR) based indices (AI) are calculated. The hydrodynamic radius (Rh) of the polymer molecules is determined using viscometry. RESULTS: For all polymers tested a linear range in the relationship AI – concentration was found. The slope of the calculated line was interpreted as measure of the molecule’s AP. The following ranking was obtained: PEG >PVP >DX >HES. Within the same chemical type of polymer, increasing Rh of the molecules leads to elevated AI. Comparison of the AP of molecules with similar Rh reveals a significant dependence on their chemical nature. CONCLUSIONS: Our results show that molecule’s AP is significantly dependent on their chemical nature – i.e. not only molecular size does matter.
