Affiliations: Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary | Department of Operative Techniques and Surgical Research, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
Note: [] Corresponding author: Norbert Nemeth M.D., Ph.D., Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Nagyerdei krt. 98., H-4032 Debrecen, Hungary. Tel./Fax: +36 52 416 915; E-mail: [email protected]
Abstract: Previously we have investigated the cerulein-induced acute pancreatitis and provided data on its micro-rheological impact in the rat. We hypothesized that non-steroid anti-inflammatory agent flunixin, the xanthine-derivate pentoxifylline and the low molecular weight heparin enoxaparin may have various beneficial effects improving microcirculatory and rheological parameters. In female rats, under general anesthesia, 10 μg/kg cerulein s.c. was administered and 2 hours afterwards microcirculation was tested by laser Doppler flowmetry on the tongue and after performing laparotomy on the small intestine, liver and pancreas prior to terminal blood sampling. From blood samples hematological parameters, blood pH, lactate concentration, erythrocyte deformability, osmoscan parameters and erythrocyte aggregation were tested. Compared to normal control in acute pancreatitis group we found severe deterioration in tissue microcirculation together with impaired erythrocyte deformability and enhanced aggregation, accompanied by acidic pH and increasing lactate concentration. Improvement was found when using flunixin (s.c.), pentoxifylline (i.p.) or enoxaparin (s.c.). These drugs could partly improve the blood flux on the surface of the investigated organs, and the flunixin had the most expressed improving effects on micro-rheological parameters. Surprisingly, the improving effect of pentoxifylline on micro-rheological parameters was not obvious (red blood cell deformability did not improved better than in the other treated groups), however, microcirculatory parameters improved.
Keywords: Red blood cell deformability, red blood cell aggregation, osmoscan, microcirculation, cerulein-induced acute pancreatitis, rat model, various drug therapy
