Gradient magnetic field effects on separation, Fourier-spectra and motion of chromatin particles and cells

Issue title: Proceedings of the twelfth International Symposium on Interdisciplinary Electromagnetic, Mechanic and Biomedical Problems, ISEM Bad Gastein

Article type: Research Article

Authors: Norina, Svetlana B.^{a; b; *} | Kim, Jungdae^a | Soh, Kwang-Sup^a

Affiliations: [a] Biomedical Physics Lab., School of Physics, Seoul National University, Seoul, 151747, Korea | [b] Physics Department, M.V. Lomonosov Moscow State University, Moscow, 119992, Russia

Correspondence: [*] Corresponding author. E-mail: [email protected]

Abstract: A magnetic capture of chromatin particles, ferritin, bacterial cells and shrimps was effectively used to measure the magnetic susceptibility, Fourier-spectra, and real-time graphs. In the high gradient magnetic separation (HGMS) using the low-frequency photo-detection the new dynamical bio-particle parameters were obtained. The concentration phase transitions and the diamagnetic alignment were revealed for colloid suspensions of the chromatin particles and the nuclei of chicken red blood cells in the HGMS. A biosensor detecting dynamical changes in the refractive index using a spectroscopy of intensity fluctuations was combined with the HGMS to detect a diamagnetic and paramagnetic capture, liquid-crystalline phase transitions in DNA containing particles, a dynamical autofluorescence and frequency spectra. Chicken red blood cell nuclei formed liquid-crystalline very bright (cholesteric) tubular-hexagonal structures and networks under their diamagnetic alignment in high gradient magnetic fields.

Keywords: High gradient magnetic separation (HGMS), diamagnetic bioparticles, chicken red blood cell nuclei, chromatin particles, ferritin nanoparticles, spectroscopy of intensity fluctuations

DOI: 10.3233/JAE-2007-881

Journal: International Journal of Applied Electromagnetics and Mechanics, vol. 25, no. 1-4, pp. 419-427, 2007