Profile of development to genes under extremely low frequency magnetic fields with caenorhabditis elegans

Issue title: Proceedings of the tenth International Symposium on Applied Electromagnetics and Mechanics ISEM-Tokyo

Article type: Research Article

Authors: Miyakawa, Tomokazu^a | Harada, Shin-ichi^b | Ishimori, Takayuki^a | Yamamoto, Hiroshi^c | Hosono, Ryuji^d | Yamada, Sotoshi^{a; *}

Affiliations: [a] Laboratory of Magnetic fields Control and Applications, Faculty of Engineering, Kanazawa University, 2-40-20 Kodatsuno, Ishikawa 920-8667, Japan | [b] Center for Biomedical Research and Education | [c] Department of Biochemistry, School of Medicine, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8640, Japan | [d] Department of Physical Information, Faculty of Medicine, Kanazawa University, 5-11-80 Kodatuno, Ishikawa 920-0942, Japan

Correspondence: [*] Corresponding author: Dr. Sotoshi Yamada, Tel.: +81 76 234 4942; Fax: +81 76 234 4946; E-mail: [email protected]

Abstract: Responses of the small heat shock protein gene, hsp-16, were examined in transgenic Caenorhabditis elegans exposed to extremly low frequency magnetic fields (ELFMFs). Expression of the hsp-16-lacZ gene was enhanced when transgenic animals were exposed to magnetic fields up to 0.5 T at 60 Hz. The hsp-16 promoter was more efficiently expressed at the embryonic than at the post-embryonic stage irrespective of exposure. Promoter activity was more sensitive to the stimulus in the intestine at the post-embryonic stage. Evidence is presented that the induction occurs at the transcriptional step of hsp-16.

DOI: 10.3233/JAE-2002-405

Journal: International Journal of Applied Electromagnetics and Mechanics, vol. 14, no. 1-4, pp. 323-326, 2002