Biofactors - Volume 11, issue 1-2

Authors: Balling, R. | Soegiarto, D.W. | Erben, R.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 1-1, 2000

Authors: Sommerburg, Olaf | Siems, Werner G. | van Kuijk, Frederik J.G.M.

Article Type: Abstract

Keywords: Lutein, zeaxanthin, carotenoids, eye, retina, macula, HPLC

Citation: Biofactors, vol. 11, no. 1-2, pp. 3-6, 2000

Authors: Tamai, Hiroshi | Kim, Han‐Suk | Hozumi, Masatoshi | Kuno, Tomoko | Murata, Takuji | Morinobu, Takao

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 7-9, 2000

Authors: Yamashita, K. | Kagaya, M. | Higuti, N. | Kiso, Y.

Article Type: Abstract

Abstract: Docosahexaenoic acid (DHA) is an essential nutrient for human health, but has extremely high oxidative susceptibility. We examined the suppressing effect of sesamin, a sesame seed lignan, on lipidperoxides in rats fed a low \alpha ‐tocopherol and high DHA containing diet. Groups of rats were fed four experimental diets: low \alpha ‐tocopherol (10 mg/kg diet) control diet, low \alpha ‐tocopherol + 0.2% sesamin diet, low \alpha ‐tocopherol + 0.5% DHA diet and low \alpha ‐tocopherol + 0.5% DHA + 0.2% sesamin diet. TBARS concentrations in plasma and …liver were significantly increased by DHA, but were completely suppressed by sesamin. \alpha ‐Tocopherol concentrations in plasma and liver decreased by addition of DHA, but with sesamin recovered to the control level. The addition of DHA into the diets caused remarkable increases of DHA concentrations in plasma and liver lipids. Sesamin caused a significant increase of DHA concentrations in the triacylglycerol of plasma. Show more

Keywords: \alpha‐Tocopherol, sesame seed, sesamin, DHA, lipid peroxide

Citation: Biofactors, vol. 11, no. 1-2, pp. 11-13, 2000

Authors: Hamre, Kristin | Berge, Rolf K. | Lie, Øyvind

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 15-17, 2000

Authors: Ciarcia, Gaetano | Paolucci, Marina | Guerriero, Giulia | Cozzolino, Giovanni | Abrescia, Paolo

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 19-21, 2000

Authors: Marczewska, J. | Koziorowska, J.H.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 23-26, 2000

Authors: Aguiló, A. | Tauler, P. | Gimeno, I. | Fuentespina, E. | Pons, A.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 27-30, 2000

Authors: Okamoto, Tadashi | Mizuta, Keiichi | Mizobuchi, Shinya | Usui, Akinori | Takahashi, Takayuki | Fujimoto, Shigeo | Kishi, Takeo

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 31-33, 2000

Authors: Cornish, Daryl A. | Maluleke, Vusi | Mhlanga, Thulani

Article Type: Abstract

Abstract: Vitamin D levels are important in the management of patients with various disorders of calcium metabolism associated with rickets, osteomalacia, osteodystrophy osteoporosis and hypoparathyroidism. 82 albinos and 58 normally pigmented children resident at the Siloe School for the Visually Impaired were sampled. Blood samples of fasting subjects were collected over a two‐day period and analyzed for vitamin D, parathyroid hormone, plasma calcium and both plasma and red blood cell magnesium measurements. The height and weight of each subject was also recorded. The results are discussed in relation to the different skin pigmented groups, for specific age groups, sex and visual …status. Statistical outliers were excluded from the results. It appears that the Albino group has significantly (p={} 0.06) higher vitamin D levels against the background of a similar dietary intake and similar exposure to sunlight/day length. Thus black children/subjects require a significantly higher intake of vitamin D to attain the same level as their Albino counterparts. In spite of significantly higher vit D levels, the other homeostatic control mechanisms were not altered (i.e., PTH levels are similar in both groups). This study supports the postulate that a dark complexion predisposes to sub‐optimal vit D status. Show more

Citation: Biofactors, vol. 11, no. 1-2, pp. 35-38, 2000

Authors: Okamoto, Kazuki | Shibata, Kiyotaka | Isohashi, Fumihide

Article Type: Abstract

Abstract: The inability to obtain sufficient quantities of purified glucocorticoid receptor (GR) causes difficulties in studying in vitro the interactions of GR with other proteins which contribute to the transcriptional activation of chromatin. Thus, we overexpressed the DNA‐binding domains (DBD) of GR in E. coli using glutathione S‐transferase (GST)‐fusion protein expression vector. The DBD‐GST protein (about 57 kDa), recovered in the soluble fraction, was purified by glutathione‐agarose column. However, during the chromatography, more than 90% of the DBD‐GST protein was cleaved into three species with molecular sizes of about 40, 35 and 30 kDa. The 40 kDa and 35 kDa proteins …were immunostained with the anti‐GR antibody, and the 30 kDa protein was stained with anti‐GST antibody. Among these proteins, only the 57 kDa DBD‐GST protein bound to the GRE‐agarose. These data indicate that the properly folded 57 kDa protein has a DNA binding function and is resistant to intrinsic cleavage of the protein. Show more

Citation: Biofactors, vol. 11, no. 1-2, pp. 39-41, 2000

Authors: Poderoso, Juan José | Boveris, Alberto | Cadenas, Enrique

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 43-45, 2000

Authors: Hernanz, A. | Fernández‐Vivancos, E. | Salazar, R.M. | Arnalich, F.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 47-49, 2000

Authors: Stabler, Sally P.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 51-52, 2000

Authors: Moiseenok, A.G. | Komar, V.I. | Khomich, T.I. | Kanunnikova, N.P. | Slyshenkov, V.S.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 53-55, 2000

Authors: Yamamoto, Shozo | Ueda, Natsuo | Mahmud, Ishtiaq | Yamaguchi, Hiroko | Yamashita, Rieko | Yamamoto, Kei | Ishimura, Kazunori | Urade, Yoshihiro | Kanaoka, Yoshihide | Hayaishi, Osamu

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 57-61, 2000

Authors: Holmgren, Arne

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 63-64, 2000

Authors: Montemartini, Marisa | Steinert, Peter | Singh, Mahavir | Flohé, Leopold | Kalisz, Henryk M.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 65-66, 2000

Authors: Guerrero, Sergio A. | Montemartini, Marisa | Spallek, Ralf | Hecht, Hans‐Jürgen | Steinert, Peter | Flohé, Leopold | Singh, Mahavir

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 67-69, 2000

Authors: Montemartini, Marisa | Nogoceke, Everson | Gommel, Daniel U. | Singh, Mahavir | Kalisz, Henryk M. | Steinert, Peter | Flohé, Leopold

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 71-72, 2000

Authors: Kalisz, Henryk M. | Hofmann, Birgit | Nogoceke, Everson | Gommel, Daniel U. | Flohé, Leopold | Hecht, Hans‐Jürgen

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 73-75, 2000

Authors: Böck, August

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 77-78, 2000

Authors: Gorlatov, Sergey N. | Stadtman, Thressa C.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 79-81, 2000

Authors: Hesse‐Bähr, K. | Dreher, I. | Köhrle, J.

Article Type: Abstract

Abstract: Metabolic labeling of HepG2 cells with ^{75} selenite indicated the expression of more than four selenoproteins in the 80 to 45 kDa range and more than four selenoproteins in the 24 to 14 kDa range. Although there were no significant changes in the total protein content, determined by the method of BioRad, the densitometric analysis of the autoradiogramms showed a reduced expression of all labeled selenoproteins in the cell lysates of HepG2 after cytokine treatment for 24 hours. A stronger reduction was observed after treatment with Il‐1\beta than with IFN\gamma . Moreover, the inhibitory effects were …more significant for those selenoproteins in the higher molecular mass range. Our data on the inhibition of selenoprotein synthesis and on repression of SeP promoter activity show that the expression of selenoproteins, especially of SeP, is influenced by acute phase reaction and pro‐inflammatory reactions. Show more

Citation: Biofactors, vol. 11, no. 1-2, pp. 83-85, 2000

Authors: Allan, Christian B.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 87-89, 2000

Authors: Kleinkauf, Horst

Article Type: Abstract

Abstract: The peptide part of CoA, 4' ‐phosphopantetheine, has been identified as an essential cofactor in in the biosynthesis of fatty acids, polyketides, depsipeptides, peptides, and compounds derived from both carboxylic and amino acid precursors, like rapamycin. The cofactor is attached to a unique protein moiety, referred to as acyl carrier protein, aminoacyl carrier protein, or peptidyl carrier protein. These carrier proteins are either associated to enzyme complexes (type II) or integrated within multifunctional polypeptide chains (type I). The cofactor is added in a post‐translational modification reaction by highly specific transferases, acting on CoA. The functions of carrier proteins in …directed condensation reactions are: (1) the selection of substrates to be attached as thioesters, (2) the stabilization of intermediates, (3) the presentation of intermediates for modification by associated enyzme activities, (4) facilitation of the directed condensation reactions of two adjacent intermediates, and (5) assistance in the termination reaction(s) leading to product release. Show more

Citation: Biofactors, vol. 11, no. 1-2, pp. 91-92, 2000

Authors: Inubushi, T. | Okada, M. | Matsui, A. | Hanba, J. | Murata, E. | Katunuma, N.

Article Type: Abstract

Abstract: When mice were placed on diets extreme deficient in vitamin B_6 , ovalbumin‐dependent antibody productions (IgE, IgG_1 , IgG_\mathrm{2a} ) were significantly suppressed, and alanine aminotransferase activity in the liver was also significantly decreased. In the case of pyridoxine excess (6 mg% = about ten times standard amount) in a 70% casein diet, ovalbumin‐dependent antibody productions were also considerably suppressed. These responses were weaker in a low casein (5%) or normal casein (20%) diet than in a 70% casein diet. The administration of high doses of pyridoxine (6 mg%) resulted in the suppression …of hepatic cathepsin B activity. Therefore, we conclude that ovalbumin‐dependent antibody productions (IgG_1 , IgE) were suppressed by pyridoxine excess diet (6 mg%), because hepatic cathepsin B activity was suppressed by the excess pyridoxine in diet. Show more

Keywords: Pyridoxine, immunoglobulin, cathepsin B, vitamin B_6

Citation: Biofactors, vol. 11, no. 1-2, pp. 93-96, 2000

Authors: Ikushiro, Hiroko | Kagamiyama, Hiroyuki

Article Type: Abstract

Abstract: Tryptophanase is catalytically more competent in alkaline pH even though the coenzyme exists as an inactive aldamine structure in this pH region. The binding of a substrate analog, 3‐indolepropionate to the enzyme shifts the equilibrium from the substituted aldamine to the ketoenamine form in the entire pH region studied. The resultant ketoenamine form is favorable for transaldimination with the substrate amino group, a prerequisite for subsequent catalysis. This implies that the binding of tryptophan in alkaline pH, where the enzyme shows maximum activity, converts the inactive aldamine form of the coenzyme to the active ketoenamine form, which is favorable for …undergoing the next step of the catalytic process. Show more

Citation: Biofactors, vol. 11, no. 1-2, pp. 97-99, 2000

Authors: Kondo, Hiroki | Kazuta, Yasuaki | Goto, Tamami

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 101-102, 2000

Authors: Katayama, Takane | Suzuki, Hideyuki | Kumagai, Hidehiko

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 103-104, 2000

Authors: Toraya, Tetsuo | Mori, Koichi | Hara, Tetsuya | Tobimatsu, Takamasa

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 105-107, 2000

Authors: Ziems, M. | Netzel, M. | Bitsch, I.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 109-110, 2000

Authors: Netzel, M. | Ziems, M. | Jung, K.H. | Noll, E. | Borsch, C. | Bitsch, I.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 111-113, 2000

Authors: Aki, Kenji | Yorita, Kazuko | Massey, Vincent

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 115-116, 2000

Authors: Matsui, A. | Tsuzuki, H. | Murata, E. | Tada, Y. | Asao, T. | Katunuma, N.

Article Type: Abstract

Abstract: We found that pridoxal phosphate (PLP), a coenzyme form of vitamin B_{6} , inhibited the activity of cathepsins B, K, S and L in vitro. Cathepsins activities in cultured splenocytes were suppressed by the addition of pridoxal (PL) or pridoxine (PI) in to the culture medium. A newly synthesized artificial vitamin B_6 derivative, a pridoxal propionate derivative, CLIK‐164, showed selective inhibition of cathepsin O/K.

Keywords: Cathepsin B, cathepsin L, cathepsin K, pridoxal, pridoxal phosphate

Citation: Biofactors, vol. 11, no. 1-2, pp. 117-120, 2000

Authors: Tanaka, Keiko | Tazuya, Keiko | Yamada, Kazuko | Kumaoka, Hiroshi

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 121-122, 2000

Authors: Yagi, Toshiharu | Ashiuchi, Makoto | Kaneda, Yasuo | Sano, Hisashi

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 123-126, 2000

Authors: Nakamura, Junko | Jung, Eun Hee | Nishino, Kohsuke

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 127-129, 2000

Authors: Dąbrowska, Ewa | Jabłońska‐Kaszewska, Irena | Łukasiak, Jerzy | Dorosz, Agnieszka | Falkiewicz, Bogdan

Article Type: Abstract

Abstract: copper

Keywords: Hepatocellular carcinoma, porphyria cutanea tarda, hemochromatosis, iron

Citation: Biofactors, vol. 11, no. 1-2, pp. 131-134, 2000

Authors: Dąbrowska, Ewa | Jabłońska‐Kaszewska, Irena | Łukasiak, Jerzy | Dorosz, Agnieszka | Falkiewicz, Bogdan

Article Type: Abstract

Keywords: Porphyria cutanea tarda, iron, zinc, copper, magnesium, liver damage

Citation: Biofactors, vol. 11, no. 1-2, pp. 135-137, 2000

Authors: Falkiewicz, Bogdan | Dąbrowska, Ewa | Łukasiak, Jerzy | Cajzer, Daniel | Jabłońska‐Kaszewska, Irena

Article Type: Abstract

Keywords: Hair analysis, metabolic X syndrome, calcium, magnesium, zinc

Citation: Biofactors, vol. 11, no. 1-2, pp. 139-141, 2000

Authors: Łukasiak, Jerzy | Jabłońska‐Kaszewska, Irena | Cajzer, Daniel | Dąbrowska, Ewa | Falkiewicz, Bogdan

Article Type: Abstract

Keywords: Hair analysis, calcium, magnesium, zinc, students

Citation: Biofactors, vol. 11, no. 1-2, pp. 143-145, 2000

Authors: Mendel, Ralf R.

Article Type: Abstract

Citation: Biofactors, vol. 11, no. 1-2, pp. 147-148, 2000