Analytical Cellular Pathology - Volume 13, issue 3

Authors: Mialhe, Agnès | Louis, Josette | Pasquier, Dominique | Rambeaud, Jean‐Jacques | Seigneurin, Daniel

Article Type: Research Article

Abstract: Recently, independent studies have shown that the expression of two integrin chains, β4 and α2, plus the epithelial cadherin are related to tumour progression in human bladder carcinomas. For the first time, we compare the expression of these three cell adhesion molecules using immunohistochemical analysis of consecutive cryosections from a series of 50 bladder tumours. E‐cadherin, β4, and α2 were strongly expressed in normal urothelium. A majority of non‐invasive bladder cancers stained positively for E‐cadherin (62%), whereas only 29% expressed normal positivity for α2, and only 35% for β4. However, most invasive tumours presented an aberrant expression of α2 (81%), …β4 (100%), and E‐cadherin (75%). We studied the correlation of immunoreactivity with histological grade and stage. The α2 pattern was not correlated with stage and grade. In contrast, loss of normal β4 expression was significantly related to increasing tumour grade and deep invasion with a higher correlation for grade. Finally, E‐cadherin expression was highly correlated with stage, but not with grade. Thus our results indicate that, although many invasive bladder tumours presented a disorder in expression of the two integrins α2 and β4, E‐cadherin appeared to be a better marker of invasiveness in bladder carcinomas. Show more

Keywords: Bladder cancer, immunohistochemistry, E‐cadherin, α2 integrin, β4 integrin

Citation: Analytical Cellular Pathology, vol. 13, no. 3, pp. 125-136, 1997

Authors: Truong, Khuong | Boenders, Jack | Maciorowski, Zofia | Vielh, Philippe | Dutrillaux, Bernard | Malfoy, Bernard | Bourgeois, Claire A.

Article Type: Research Article

Abstract: The purpose of this study was to improve the detection of FISH signals, in order that spot counting by a fully automated image cytometer be comparable to that obtained visually under the microscope. Two systems of spot scoring, visual and automated counting, were investigated in parallel on stimulated human lymphocytes with FISH using a biotinylated centromeric probe for chromosome 3. Signal characteristics were first analyzed on images recorded with a coupled charge device (CCD) camera. Number of spots per nucleus were scored visually on these recorded images versus automatically with a DISCOVERY image analyzer. Several fluochromes, amplification systems and pretreatments …were tested. Our results for both visual and automated scoring show that the tyramide amplification system (TSA) gives the best amplification of signal if pepsin treatment is applied prior to FISH. Accuracy of the automated scoring, however, remained low (58% of nuclei containing two spots) compared to the visual scoring because of the high intranuclear variation between FISH spots. Show more

Keywords: FISH, interphase cytogenetics, image analysis, spot counting, signal amplification

Citation: Analytical Cellular Pathology, vol. 13, no. 3, pp. 137-146, 1997

Article Type: Abstract

Citation: Analytical Cellular Pathology, vol. 13, no. 3, pp. 147-201, 1997

Article Type: Other

Citation: Analytical Cellular Pathology, vol. 13, no. 3, pp. 203-204, 1997