Investigation of Interaction between Pax-5 Isoforms and Thioredoxin Using De Novo Modelling Methods

Article type: Research Article

Authors: Cuperlovic-Culf, Miroslava | Robichaud, Gilles A. | Nardini, Michel^; | Ouellette, Rodney J.^;

Affiliations: Laboratorie de pathologie moléculaire, Institut de recherche médicale Beauséjour, 35 Providence Street, Moncton, NB. E1C 8X3, Canada | Départment de chimie et biochimie, Université de Moncton, Moncton, N.-B., Canada

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Abstract: The Pax-5 transcription factor plays a crucial role in B-cell development, activation and differentiation. In murine B-cells four different isoforms of Pax-5 have been identified, and their role in the regulation of the activity of the wild-type protein was revealed although still not fully understood. Using theoretical methods, we investigated the properties of one region of the Pax-5e and Pax-5d isoforms (named UDE domain) and we present a possible theoretical model for the interaction of this domain with thioredoxin that have been previously postulated based on the experimental results. Domain UDE (MW 4.8kDa) is characterised by an extremely high ratio of positively charged residues (8) in comparisons to negatively charged amino acids (3), as well as unusually large concentrations of prolines (11.6%) and cysteines (4.7%). This is indicative of its role in protein-protein interaction. The experimental 3D structure for either UDE domain or for any analogous sequence is not yet available, and therefore we resorted to various bioinformatics methods in order to predict the secondary and 3D structure from the primary sequence of UDE. Physicochemical properties of the predicted UDE structure gave more indication about possibilities for UDE-thioredoxin binding. In addition, UDE domain was shown to have both sequence and structure analogous to a segment of NAD-reducing hydrogenase HOXS α subunit which is believed to interact with thioredoxin. These studies showed that the UDE domain in Pax-5d and Pax-5e represents an ideal binding site for thioredoxin and we developed a model of UDE-TRX complex with two disulphide bridges. The active site of thioredoxin remained exposed after binding to UDE in this model and therefore binding of thioredoxin to Pax-5d could explain the unexpectedly high resistance of this isoform to oxidation. The complex between thioredoxin and Pax-5e can be a method for transportation of thioredoxin into the nucleus and also into the the vicinity of Pax-5a, explaining the observed activator role of Pax-5e.

Keywords: protein structure, protein structure prediction, protein-protein interaction, molecular modelling, bioinformatics, transcription factors, alternative splicing, HMMSTR, Rosetta, theoretical analysis, proteomics, electrostatics, disulphide bridge calculation

Journal: In Silico Biology, vol. 3, no. 4, pp. 453-469, 2003