Immunocytochemical detection of lung cancer cells with monoclonal antibodies to 14-3-3 proteins

Article type: Research Article

Authors: Setoguchi, Yuko^{a; *} | Kato, Masatoshi^a | Shoji, Masahiro^a | Honjoh, Tsutomu^a | Kamei, Masanori^a | Sugitani, Masanori^a | Sato, Susumu^a | Hashizume, Shuichi^a | Hanagiri, Takeshi^b | Yoshimatsu, Takashi^b | Nakanishi, Kozo^b | Yasumoto, Kosei^b | Nagashima, Akira^c | Nakahashi, Hisashi^d | Suzuki, Tatsuo^e | Imai, Tadaaki^f | Shirahata, Sanetaka^g | Nomoto, Kikuo^h

Affiliations: [a] Morinaga Institute of Biological Science, Tsurumi-ku, Yokohama | [b] Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu | [c] Department of Chest Surgery, Kitakyushu Municipal Medical Center, Kokurakita-ku, Kitakyushu | [d] Matsuyama Red Cross Hospital, Matsuyama | [e] Department of Research Planning, Kitasato Institute Hospital, Minato-ku, Tokyo | [f] Section of Pathology, The Kitasato Institute Medical Center Hospital, Kitamoto, Saitama | [g] Graduate School of Genetic Resources Technology, Kyushu University, Higashi-ku, Fukuoka | [h] Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka, Japan

Correspondence: [*] Correspondence and reprint requests to: Yuko Setoguchi, Morinaga Institute of Biological Science 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230, Japan

Abstract: Murine monoclonal antibodies were raised against 14-3-3 proteins, the antigen of human monoclonal antibody AE6F4 which had been shown potentially useful for the immunochemical diagnosis of lung cancer via sputum cytology. Enzyme-linked immunosorbent assays of the murine anti-14-3-3 monoclonal antibodies with isolated bovine brain 14-3-3 isoforms showed that the antibodies were classified into four different profiles of isoform reactivity. The comparison of 14-3-3 isoform and lung cancer tissue on the reactivity with murine monoclonal antibodies indicated that β isoform can be responsible for cancer recognition, whereas human monoclonal antibody AE6F4 showed preferential binding to ζ isoform. No murine monoclonal antibody of the same isoform specificity as human monoclonal antibody AE6F4 was obtained. Since murine monoclonal antibodies with different isoform specificities could immunostain lung cancer cells in sputum successfully, the combination use of murine monoclonal anti-14-3-3 antibodies with human monoclonal antibody AE6F4 is potentially usefol for facilitating the sputum cytodiagnosis of lung cancer.

Keywords: Human MAb AE6F4, murine anti-14-3-3 MAbs, 14-3-3 isoforms, cancer recognition, sputum cytodiagnosis, lung cancer

DOI: 10.3233/HAB-1995-6403

Journal: Human Antibodies, vol. 6, no. 4, pp. 137-144, 1995