Affiliations: [a] Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran | [b] Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran | [c] Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Shahid Labbafi Nejad Educational Hospital, Tehran, Iran
Correspondence:
[*]
Corresponding author: Arezou Sayad, Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, PO Box 1985717443, Tehran, Iran. Tel.: +98 21 23872572; Fax: +98 2123872572; E-mail:[email protected]
Note: [1] Mohammad Taheri is equally first author to Zahra Rezaie.
Abstract: BACKGROUND: Multiple sclerosis (MS) as a complex neurological
disease can be due to vitamin D deficiency. CYP27B1 is referred to as a
vitamin D metabolizing enzyme. MATERIALS AND METHODS: This study
compared the expression level of CYP27B1 in Relapsing-Remitting MS (RRMS)
patients with normal individuals in Iran. The RNA was extracted from 50 RRMS
patients and 50 normal controls. Quantitative RT-PCR was adopted to measure
the expression level of CYP27B1 gene. RESULTS: The expression level
of CYP27B1gene was significantly lower in the RRMS patients than their
normal counterparts (P value = 0.04). Also, the RRMS females participating had
a significant reduction in CYP27B1 gene expression compared to normal
females (P-Value = 0.01). In addition, the correlation between CYP27B1
expression level, and the risk of Expanded Disability Status Scale
of Kurtzke (EDSS) was not linear. Additionally, there was no significant
correlation between expression status of CYP27B1gene and duration of the
disease. CONCLUSION: A significant decrease in the expression level
of CYP27A1 in female patients could indicate their greater vulnerability to
MS than the male patients.
Keywords: CYP27B1, expression, multiple sclerosis, real time PCR
