Evaluating the effect of siRNA on SOX2OT expression in the human neuron-committed teratocarcinoma NT2 cell line

Article type: Research Article

Authors: Sadeghi, Zahra^a | Dodange, Fateme^a | Maleki, Parichehr^a | Zarei, Mohadeseh^a | Taheri, Mohammad^b | Raheb, Jamshid^{a; *}

Affiliations: [a] National Institute of Genetic Engineering and Biotechnology, Tehran, Iran | [b] Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Correspondence: [*] Corresponding author: Jamshid Raheb, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. E-mail: [email protected].

Abstract: Non-coding RNA elongated (lncRNAs) have recently attracted as molecules that regulate gene expression of the pluripotent properties (pluripotency) of stem cells. Recently our colleagues examined the role of one of these RNAs called SOX2OT in esophageal squamous cell carcinoma, and found a concomitant increase in its expression with some regulatory genes of cell proliferation. In the present study, using the design of suitable primers from SOX2OT gene, we investigated the effect of siRNA on expression of SOX2OT.

Keywords: NT2, siRNA, SOX2OT

DOI: 10.3233/HAB-200418

Journal: Human Antibodies, vol. 28, no. 4, pp. 299-303, 2020