Affiliations: [a] Applied Medical Chemistry Department, Medical Research Institute,
Alexandria University, Alexandria, Egypt | [b] Microbiology Department, Medical Research Institute,
Alexandria University, Alexandria, Egypt | [c] Pathology Department, Medical Research Institute,
Alexandria University, Alexandria, Egypt | [d] Surgery Department, Medical Research Institute,
Alexandria University, Alexandria, Egypt | [e] Microbial Biotechnology Department,
National Research Centre, Giza, Egypt | [f] Medical Laboratory Technology Department,
College of Applied Medical Sciences, Jazan University,
Jazan, Saudi Arabia | [g] Internal Medicine Hospital, Faculty of Medicine,
Mansoura University, Mansoura, Egypt
Correspondence:
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Corresponding author: Ehab Y. Elbendary, Medical Laboratory Technology Department, College of Applied Medical %****␣hab-27-hab190372_temp.tex␣Line␣25␣**** Sciences, Jazan University, Jazan, Saudi Arabia. Tel.: +96 654 645 2214; E-mail: [email protected].
Abstract: BACKGROUND: The Lewis (b) blood group antigen-Binding Adhesion2 (BabA2) has been reported to mediate the attachment of H. pylori to human. AIM: assessment the diagnostic potential of detection of (BabA2) gene compared with immunostaining of Lewis (b) by specific mouse monoclonal antibodies in gastric biopsies from Egyptian Patients as a diagnostic maker for Helicobacter pylori infection. MATERIALS AND METHODS: Fifty untreated patients suffering from dyspeptic complaints were enrolled in this study and underwent for upper gastro-duodenal endoscopy. Biopsies were taken for histological examination by (H&E) and immunohistochemical analysis for Lewis b by specific mouse monoclonal antibodies, and scoring of Lewis b expression in gastric tissue biopsy as well as molecular detection of BabA2 gene of H. pylori by PCR. Biochemical analysis was performed to detect the presence of H. pylori urease activity using Rapid Urease Test (RUT). RESULTS: Out of 50 gastric biopsies, 41 biopsies were positive for histological, Immunostaining for Lewis b expression and urease activity test (RUT) for H pylori. RUT showed a sensitivity of 87.8%, specificity 88.9%, positive predictive value (PPV) 97.2%, and negative predictive value (NPV) 61.5%. BabA2 gene results revealed that, out of 41 positive biopsied cases, 39 (95.1%) were positive by the PCR test for BabA2 gene. And all 9 negative biopsies (100%) for H pylori negative for BabA2gene so the sensitivity and specificity of BabA2 gene detection in gastric biopsies by PCR were 95.1% and 100%; respectively. CONCLUSION: BabA2 gene detection in gastric tissue biopsies could be suggested as a diagnostic biomarker to be included among the other biomarkers routinely performed for clinical diagnosis of H. pylori infection.
Keywords: Helicobacter pylori, polymerase chain reaction, immunohistochemistry, Lewis b expression BabA2, genotype
