Affiliations: [a] Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | [b] Graduated Student of Microbiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | [c] Department of Venomous Animals and Toxins, Razi Vaccine and Serum Research Institute, Ahvaz Branch, Agriculture Research Education and Extension Organization (AREEO), Iran
Correspondence:
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Corresponding author: Mohammad Khosravi, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Khuzestan, Ahvaz, Iran. E-mail: [email protected].
Abstract: BACKGROUND: The hypersensitivity reaction to penicillin is a public health problem. Immunological responses to penicillin and other beta-lactam antibiotics can be classified into immediate and non-immediate responses. The immediate hypersensitivity is mediated by IgE; however, the non-immediate sensitivity is facilitated by other isotypes of antibody or T lymphocytes. OBJECTIVE: This research detected the non-IgE antibody value against penicillin in allergic and normal people. METHODS: Thirty-eight samples from patients with positive or negative intradermal skin testing results of penicillin allergy were included in this study. The total antibody and IgM levels against penicillin G were defined by in-house ELISA test. RESULTS: The results showed a significant (P< 0.05) elevation in total immunoglobulin and non-IgM anti-penicillin antibody of sensitive groups; however, the anti-penicillin IgM was significantly greater in non-sensitive peoples. CONCLUSIONS: Although the sensitized people to penicillin cannot be certainly detected with the total antibody, specific IgG and IgM value against penicillin, these values are good indicators for prediction of immediate and late response of the immune system to penicillin.
Keywords: Penicillin, sensitivity, antibody, ELISA, human
