Affiliations: Department of Medical Immunology, Medical School (Charité), Humboldt University Berlin, GDR
Note: [] Address reprint requests to: Dr. R.W. Glaser, Inst. Med. Immunologie, Schumannstr. 20/21, Berlin, DDR-1020, GDR. A version of this paper was presented at the First International Conference on Human Antibodies and Hybridomas, Orlando, FI, USA, 18–20 April 1990.
Abstract: Human lymphocytes from peripheral blood (MNC) were separated on magnetic beads for the presence of different surface markers. Cells from positive and negative fractions were successfully immortalized by electro fusion with the heteromyeloma line CB-Fu2. B cells, which were separated on anti-CD 19 coated beads, could be immortalized at a rate between 10−5 and 10−4 even if the fusion was conducted with just a few hundred thousand cells. Comparison of the frequency of 19-positive hybridomas in B cell, T cell, and unseparated MNC fusions indicated that also non-B cells may give rise to HAT resistant hybridoma clones, although the fusion frequency was low.
Keywords: electrofusion, human mononuclear cells, B cells, T cells
