Affiliations: Sandoz Research Institute, East Hanover, New Jersey, USA
Note: [] Address requests for reprints to: Paul H. Ehrlich, Ph.D., Sandoz Research Institute, Sandoz Pharmaceuticals Corporation, Rt. 10, E. Hanover, NJ 07936, USA.
Abstract: The nucleotide sequence of the mRNA that codes for Fab fragments from two chimpanzee monoclonal antibodies has been determined. Both antibodies have high affinity and good specificity for digoxin. Four domains from the two antibodies have been sequenced: the constant domains of the kappa and lambda light chains, the variable domain of the lambda light chain, and the CHl domain of the IgG1 heavy chain. There are very few differences between the chimpanzee and human sequences; the nucleotide sequences differ by less than 2%.
