Biochemical and immunological properties of four intravenous Immunoglobulin G preparations

Article type: Research Article

Authors: Redwan, EL-Rashdy M.^{; *}

Affiliations: Protein Research Department, GEBRI, Mubarak City for Scientific Research and Biotechnology application, New Bor El-Arab, Alexandria, Egypt. E-mail: [email protected]

Note: [*] Present address: The Scripps Research Institute, Department of Molecular Biology BCC206, 10550 N.Torrey Pines Rd., La Jolla CA 92037, USA. E-mail: [email protected]

Abstract: Intravenous Immunoglobulin G (IVIG) is one of the most important and widely used human plasma fractions. Although of this there are some precautions should be considered to use this product; e.g. biochemical, biological, immunological, and safety (virus transmission) for each produce patch. The aim of this study was to investigate four commercial IVIG preparations used in Egyptian hospitals. The results show that IVIG from Korean Green Cross Cooperation and Octapharma contained 100% intact IgG, while IVIG-VACSERA and VIGAM-S contained 98--99% IgG subclass distribution corresponded to the distribution in normal human plasma, except for IVIG-VACSERA, where IgG2 slightly elevated. IVIG-VACSERA and VIAGM-S contained acceptable traces of IgA/IgM, while preparations from both Korean Green Cross Cooperation and Octapharma contained 130/150 and 100/80 μg/ml, respectively. Each preparation had a strong potential to react with respiratory and enteric bacterial LPS and tetanus toxoid antigen. Our results are in general agreement with previous status of worldwide diphtheria immunity status. The immunoreactivity with the other antigen will discuss.

DOI: 10.3233/HAB-2002-11304

Journal: Human Antibodies, vol. 11, no. 3, pp. 79-84, 2002