Affiliations: Department of Oncology and Pathology, Karolinska Institute, Stockholm, Sweden | Department of Oncology, KFC, Huddinge University Hospital, Sweden | Department of Veterinary Medical Chemistry, Swedish University of Agricultural Science, Biomedical Center, Uppsala, Sweden
Note: [] Corresponding author: Dr. Bernhard Tribukait, Department of Oncology and Pathology, Karolinska Institute, CCK R8:00, 171 76, Stockholm, Sweden. Tel.: +46 8 5177 4361; Fax: +46 8 517 75042; E‐mail: [email protected].
Abstract: The cytosolic thymidine kinase 1 (TK1) is one of the enzymes involved in DNA replication. Based on biochemical studies, TK1 is activated at late G1 of cell cycle, and its activity correlates with the cell proliferation. We have developed a polyclonal anti‐TK1 antibody against a synthetic peptide from the C‐terminus of human TK1. Using this antibody, here we demonstrate the exclusive location of TK1 in the cytoplasm of cells. Cell cycle dependent TK1 expression was studied by simultaneous fluorescence staining for TK1 and bromodeoxyuridine, by using elutriated cells, and by quantitation of the amount TK1 in relation to the cellular DNA content. TK1, which was strongly expressed in the cells in S+G2 period, raised at late G1 and decreased during mitosis. The amount of TK1 increased three folds from late G1 to G2. TK1 positive cells were demonstrated in areas of proliferation activity of various normal and malignant tissues. The new anti‐TK1 antibody works in archival specimens and is a specific marker of cell proliferation.
