Consensus report of the task force on standardisation of DNA flow cytometry in clinical pathology

Article type: Research Article

Authors: Ormerod, M.G.^; | Tribukait, B. | Giaretti, Walter^;

Affiliations: 34 Wray Park Road, Reigate, RH2 ODE, UK | Department of Medical Radiobiology, Karolinska Institute, Box 60212, S‐104 01 Stockholm, Sweden | Laboratory of Biophysics, Istituto Nazionale per la Ricera sul Cancro, Viale Bendetto XV, n. 10, 16132 Genova, Italy

Note: [] Corresponding author: Michael Ormerod. E‐mail: [email protected].

Note: [] On behalf of the DNA Flow Cytometry Task Force of the European Society for Analytical Cellular Pathology. Members of the group: A. Böcking, Institut für Cytopatholgie, Medizinische Einrichtungen, Heinrich Heine Universität, Düsseldorf, Germany; I. Christensen, Finsen Laboratory, Rigshospitalet, Copenhagen, Denmark; J.‐L. D’Hautcourt, Clinique St. Joseph, Mons, Belgium; J. Dufer, Laboratoire de Physiologie Cellulaire, Université de Reims, Reims, France; W. Giaretti, Laboratory of Biophysics, Istituto Nazionale per la Ricera sul Cancro, Genova, Italy; J. Lawry, Institute of Cancer Studies, Medical School, Sheffield, UK; A. Orfao, Servicio General de Citometria, Laboratorio de Hematologia, Hospital Clinico Universitario, Salamanca, Spain; M.G. Ormerod, 34 Wray Park Road, Reigate, UK; A. Sampedro, Servicio de Proceso de Imagenes, Universidad de Oviedo, Oviedo, Spain; F. Sansonetty, IPATIMUP, Porto, Portugal; B. Tribukait, Karolinska Institute, Stockholm, Sweden; G.K. Valet, Max‐Planck‐Institut für Biochemie, Cell Biochemistry Laboratory, Martinsried, Germany.

Abstract: Guidelines are given to assist the standardisation of DNA flow cytometry in clinical pathology. They have been agreed by a group of twelve scientists from nine European countries.

Keywords: DNA, flow cytometry, standardisation, ploidy, S‐phase fraction

Journal: Analytical Cellular Pathology, vol. 17, no. 2, pp. 103-110, 1998